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[气相色谱法测定挥发性脂肪酸(C1 - C5),包括经苯基重氮甲烷转化为其苄酯后的乳酸。青贮饲料中酸的测定(作者译)]

[Gas chromatographic determination of the volatile fatty acids (C1-C5) including lactic acid after conversion into their benzyl esters by phenyldiazomethane. Determination of the acids in silages (author's transl)].

作者信息

Doms E K

出版信息

J Chromatogr. 1977 Oct 1;140(1):29-39. doi: 10.1016/s0021-9673(00)83598-0.

Abstract

A gas chromatographic method for the determination of the volatile fatty acids (C1-C5) including lactic acid is described. The acids are converted into their benzyl esters by means of phenyldiazomethane without previous purification of the reagent by distillation. The benzyl esters are well separated from the solvent as well as from one another. Silicone SE-30 is used as the stationary phase. An application of the method on silage acids is demonstrated.

摘要

描述了一种用于测定包括乳酸在内的挥发性脂肪酸(C1 - C5)的气相色谱法。通过苯基重氮甲烷将这些酸转化为它们的苄酯,无需事先通过蒸馏对试剂进行纯化。苄酯与溶剂以及它们彼此之间都能很好地分离。使用硅酮SE - 30作为固定相。展示了该方法在青贮饲料酸中的应用。

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