Blay Karine Le, Caroff Martine, Blanchard Frédéric, Perry Malcolm B, Chaby Richard
Equipe 'Endotoxines', URA-1116 du CNRS, Université de Paris-Sud, 91405 Orsay, France.
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
Microbiology (Reading). 1996 Apr;142 ( Pt 4):971-978. doi: 10.1099/00221287-142-4-971.
Three hybridomas (P1P3, D7 and 60.5) producing monoclonal antibodies (mAbs) against Bordetella pertussis lipopolysaccharide (LPS) were established. All reacted with the LPS from a typical, vaccine strain of B. pertussis (1414), but not with that of a variant strain (A100). Two of these mAbs (P1P3 and 60.5) cross-reacted with a B. bronchiseptica LPS; only one (P1P3) reacted with a B. parapertussis LPS. ELISA reactivities with intact LPSs, and defined partial structures covalently linked to bovine serum albumin, were compared. mAb 60.5 bound to the terminal region of a distal trisaccharide consisting of N-acetylated amino sugars. D7 reacted with a substructure which can be modified in the B. parapertussis and B. bronchiseptica LPSs by addition of a polymeric O-chain. P1P3 bound to a nonacetylated glucosamine substituted with L-glycero-D-manno-heptose, present in the 'core' of the B. pertussis LPS. These mAbs may be useful for rapid typing of Bordetella in clinical isolates.
建立了三种产生抗百日咳博德特氏菌脂多糖（LPS）单克隆抗体（mAb）的杂交瘤（P1P3、D7和60.5）。所有杂交瘤都与典型的百日咳博德特氏菌疫苗株（1414）的LPS发生反应，但不与变异株（A100）的LPS反应。其中两种mAb（P1P3和60.5）与支气管败血博德特氏菌LPS发生交叉反应；只有一种（P1P3）与副百日咳博德特氏菌LPS反应。比较了完整LPS以及与牛血清白蛋白共价连接的特定部分结构的ELISA反应性。mAb 60.5与由N-乙酰化氨基糖组成的远端三糖的末端区域结合。D7与一种亚结构反应，该亚结构在副百日咳博德特氏菌和支气管败血博德特氏菌LPS中可通过添加聚合O链进行修饰。P1P3与百日咳博德特氏菌LPS“核心”中存在的被L-甘油-D-甘露庚糖取代的非乙酰化葡糖胺结合。这些mAb可能有助于对临床分离株中的博德特氏菌进行快速分型。
Zotero 插件