Amano K, Fukushi K, Watanabe M
Department of Bacteriology, Hirosaki University School of Medicine, Aomori, Japan.
J Gen Microbiol. 1990 Mar;136(3):481-7. doi: 10.1099/00221287-136-3-481.
Lipopolysaccharides (LPS) isolated from Bordetella pertussis, B. parapertussis and B. bronchiseptica were analysed for their chemical composition, molecular heterogeneity and immunological properties. All the LPS preparations contained heptose, 3-deoxy-D-manno-2-octulosonic acid, glucosamine, uronic acid, phosphate and fatty acids. The fatty acids C14:0, C16:0 and beta OHC14:0 were common to all the LPS preparations. LPS from B. pertussis strains additionally contained isoC16:0, those from B. parapertussis contained isoC14:0 and isoC16:0, and those from B. bronchiseptica contained C16:1. By SDS-PAGE, LPS from B. pertussis had two bands of low molecular mass, and the LPS from B. parapertussis and B. bronchiseptica showed low molecular mass bands together with a ladder arrangement of high molecular mass bands. Immunodiffusion, quantitative agglutination and ELISA demonstrated that the LPS from B. pertussis strains reacted with antisera prepared against whole cells of B. pertussis and B. bronchiseptica; LPS from B. parapertussis reacted with antisera to B. parapertussis and B. bronchiseptica, and LPS from B. bronchiseptica reacted with anti-whole cell serum raised against any of the three species. From these results, it is concluded that LPS from B. bronchiseptica has structures in common with LPS from B. pertussis and B. parapertussis, while the LPS from B. pertussis and B. parapertussis are serologically entirely different from each other.
对从百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血博德特氏菌中分离出的脂多糖（LPS）进行了化学成分、分子异质性和免疫学特性分析。所有LPS制剂均含有庚糖、3-脱氧-D-甘露-2-辛酮糖酸、葡糖胺、糖醛酸、磷酸盐和脂肪酸。脂肪酸C14:0、C16:0和β-OHC14:0在所有LPS制剂中都很常见。百日咳博德特氏菌菌株的LPS还含有异C16:0，副百日咳博德特氏菌的LPS含有异C14:0和异C16:0，支气管败血博德特氏菌的LPS含有C16:1。通过SDS-PAGE分析，百日咳博德特氏菌的LPS有两条低分子量条带，副百日咳博德特氏菌和支气管败血博德特氏菌的LPS显示出低分子量条带以及高分子量条带的阶梯状排列。免疫扩散、定量凝集和ELISA表明，百日咳博德特氏菌菌株的LPS与针对百日咳博德特氏菌和支气管败血博德特氏菌全细胞制备的抗血清发生反应；副百日咳博德特氏菌的LPS与针对副百日咳博德特氏菌和支气管败血博德特氏菌的抗血清发生反应，支气管败血博德特氏菌的LPS与针对这三种菌中任何一种制备的抗全细胞血清发生反应。从这些结果可以得出结论，支气管败血博德特氏菌的LPS与百日咳博德特氏菌和副百日咳博德特氏菌的LPS具有共同结构，而百日咳博德特氏菌和副百日咳博德特氏菌的LPS在血清学上彼此完全不同。
