Gustafsson B, Lindquist U, Andersson M
Department of Vaccine Production, National Bacteriological Laboratory, Stockholm, Sweden.
J Clin Microbiol. 1988 Feb;26(2):188-93. doi: 10.1128/jcm.26.2.188-193.1988.
Hybrid cell lines producing monoclonal antibodies against Bordetella pertussis lipopolysaccharide (LPS) were established. The specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay (ELISA) and ELISA-inhibition experiments with LPS and delipidated polysaccharide fragments (PS-1 and PS-2) prepared from B. pertussis LPS. Monoclonal antibody 9-1-H5 reacted with B. pertussis LPS only, whereas monoclonal antibodies 6-4-H6 and 9-2-A8 reacted with PS-1 and PS-2 as well as B. pertussis LPS. The antibodies did not react with LPS prepared from B. parapertussis and B. bronchiseptica in an LPS-specific ELISA. A monoclonal antibody-based sandwich ELISA was developed for detection of B. pertussis LPS. This assay had a detection limit of B. pertussis LPS in concentrations ranging from 0.16 to 0.32 microgram/ml. The assay was also shown to be specific for the detection of whole B. pertussis bacteria. No cross-reactions were observed with strains of Branhamella catarrhalis, Neisseria meningitidis, Streptococcus miteor, Haemophilus influenzae, or Legionella pneumophila. The monoclonal antibodies might be useful for the detection of soluble antigens and whole bacteria in clinical samples and for studies of the immunochemical structure of B. pertussis LPS.
建立了产生抗百日咳博德特氏菌脂多糖（LPS）单克隆抗体的杂交细胞系。通过酶联免疫吸附测定（ELISA）以及使用从百日咳博德特氏菌LPS制备的LPS和脱脂多糖片段（PS-1和PS-2）进行的ELISA抑制实验来确定抗体的特异性。单克隆抗体9-1-H5仅与百日咳博德特氏菌LPS反应，而单克隆抗体6-4-H6和9-2-A8与PS-1、PS-2以及百日咳博德特氏菌LPS都反应。在LPS特异性ELISA中，这些抗体不与副百日咳博德特氏菌和支气管败血博德特氏菌制备的LPS反应。开发了一种基于单克隆抗体的夹心ELISA用于检测百日咳博德特氏菌LPS。该测定法对百日咳博德特氏菌LPS的检测限为0.16至0.32微克/毫升。该测定法还显示对检测完整的百日咳博德特氏菌具有特异性。未观察到与卡他布兰汉菌、脑膜炎奈瑟菌、微小链球菌、流感嗜血杆菌或嗜肺军团菌菌株的交叉反应。这些单克隆抗体可能有助于检测临床样本中的可溶性抗原和完整细菌，以及用于百日咳博德特氏菌LPS免疫化学结构的研究。
