Wu F F, Dobberstein R H
J Chromatogr. 1977 Oct 1;140(1):65-70. doi: 10.1016/s0021-9673(00)83602-x.
A method is described for the quantitative analysis of thebaine from Papaver bracteatum, using a single high-pressure liquid chromatographic column. The procedure gives base-line separation of thebaine without the need for gradient elution equipment, and can be completed within 12 min. Thebaine isolated by this technique was shown to be pure, regardless of the age of plant or plant part from which it was obtained.
描述了一种使用单一高压液相色谱柱对紫罂粟中的蒂巴因进行定量分析的方法。该方法无需梯度洗脱设备即可实现蒂巴因的基线分离,且可在12分钟内完成。无论从何种年龄的植物或植物部位获得蒂巴因,通过该技术分离出的蒂巴因均显示为纯净的。