Kirkland J J, Yau W W, Stoklosa H J, Dilks C H
J Chromatogr Sci. 1977 Aug;15(8):303-16. doi: 10.1093/chromsci/15.8.303.
Chromatographic resolution and analysis accuracy can be seriously affected by broadening of very sharp bands associated with both high-performance size-exclusion chromatography and other liquid chromatography methods using less than 5 micron particles. The effect on column plate count by extra-column band broadening from injection systems, detectors, connectors and "guard" columns is discussed and exemplified with experimental data. The special cases of tailing and doublet peaks for a single species are investigated. These effects are eliminated by a special sample injection technique. Computer simulation studies demonstrated that serious errors can result from calculating column plate count with non-Gaussian peaks. The maximum allowable peak tailing is suggested and a new method for quantitating peak skew is proposed.
高效尺寸排阻色谱法以及使用小于5微米颗粒的其他液相色谱方法中，非常尖锐的谱带变宽会严重影响色谱分辨率和分析准确性。本文讨论了进样系统、检测器、连接管和“保护”柱产生的柱外谱带展宽对柱板数的影响，并给出实验数据示例。研究了单一物质出现拖尾峰和双峰的特殊情况。采用一种特殊的进样技术可消除这些影响。计算机模拟研究表明，使用非高斯峰计算柱板数会导致严重误差。文中建议了最大允许峰拖尾，并提出了一种定量峰不对称性的新方法。
