兔腺病毒5型感染的眼部模型。
An ocular model of Adenovirus type 5 infection in the rabbit.
作者信息
De Hauwere B, Lutz A, Maudgal P C, Neyts J, de Clercq E
机构信息
Dienst Oogheelkunde, U.Z. Leuven.
出版信息
Bull Soc Belge Ophtalmol. 1995;259:33-42.
Adenoviruses are a common cause of viral eye disease in humans. Recently, new antiviral drugs have been developed, which possess promising anti-adenovirus activity. In order to test these drugs, an ocular model of adenovirus infection was developed in 10 Hollander rabbits following topical, intrastromal and subconjunctival inoculation with a standard laboratory serotype (Adenovirus type 5 from The American Type Culture Collection). Clinical signs of infection-conjunctivitis, keratitis, corneal edema, subepithelial opacities, anterior chamber reaction and iritis-were evaluated. Adenovirus was isolated from the eye several days after inoculation and intra-epithelial viral replication was detected using the replica-technique. Reproducible ocular infection was clinically demonstrated in all rabbits.
腺病毒是人类病毒性眼病的常见病因。最近，已开发出具有良好抗腺病毒活性的新型抗病毒药物。为了测试这些药物，在用标准实验室血清型（来自美国模式培养物集存库的5型腺病毒）进行局部、基质内和结膜下接种后，在10只荷兰兔中建立了腺病毒感染的眼部模型。对感染的临床体征——结膜炎、角膜炎、角膜水肿、上皮下混浊、前房反应和虹膜炎——进行了评估。接种几天后从眼中分离出腺病毒，并使用复制技术检测上皮内病毒复制。所有兔子均在临床上表现出可重复的眼部感染。
Zotero 插件