Tsurushima H, Tsuboi K, Yoshii Y, Ohno T, Meguro K, Nose T
Department of Neurosurgery, Tsukuba Medical Center Hospital, Ibaraki, Japan.
Neurol Med Chir (Tokyo). 1996 Oct;36(10):704-8. doi: 10.2176/nmc.36.704.
Expression of N-ras gene was examined by northern blot analysis in two cases of astrocytoma (grade II), four cases of glioblastoma multiforme (six samples including two recurrent tumors), and normal brain tissues. Expression of N-ras gene was only found in the two recurrent glioblastoma multiforme samples. Southern blot analysis found no significant difference in copy numbers of N-ras gene in pre- and post-recurrence samples. The histological diagnosis of the two recurrent tumors was glioblastoma multiforme at both first recurrent presentation. However, the tumors had progressed during recurrence, because of worsened histological findings, such as increased cellularity, pleomorphism, and vascularity. These tumors recurred at very short intervals. Enhanced expression of N-ras gene by disorder of transcription may be a factor in the progression of glioblastoma multiforme.
通过Northern印迹分析检测了2例星形细胞瘤（II级）、4例多形性胶质母细胞瘤（6个样本，包括2例复发性肿瘤）以及正常脑组织中N-ras基因的表达。仅在2例复发性多形性胶质母细胞瘤样本中发现了N-ras基因的表达。Southern印迹分析发现复发前后样本中N-ras基因的拷贝数无显著差异。这2例复发性肿瘤在首次复发时的组织学诊断均为多形性胶质母细胞瘤。然而，由于组织学表现恶化，如细胞增多、多形性和血管形成增加，肿瘤在复发期间出现了进展。这些肿瘤复发间隔非常短。转录紊乱导致的N-ras基因表达增强可能是多形性胶质母细胞瘤进展的一个因素。
