Tani A, Tatsumi E, Nakamura F, Kumagai S
Department of Laboratory Medicine, Kobe University School of Medicine.
Rinsho Byori. 1996 Oct;44(10):936-43.
The YT cells, already known as natural killer (NK) line, were tested for Epstein-Barr virus (EBV). The simply maintained YT cells (YT-O) and the two different subclones showed and identical length of junctional DNA of terminal repeats in Southern blot with LMP-1 probe, indicating that the 3 had already been positive for EBV before subcloning. YT-O expressed limited amount of EBNA2 or LMP-1 mRNA, whereas the 2 subclones expressed abundant EBNA2 or LMP-1 mRNA in the Northern blot analysis with EBNA2 or LMP-1 probe. Thus, the ex vivo cells were positive for EBV, since the BL (Burkitt lymphoma) type EBV gene expression in the YT-O does not generally occur in in vitro infection. The remaining clinical record indicated that the lymphoma was extranodal (angiocentric lymphoma), involving mediastinum and liver, but not nodal or lymphoblastic lymphoma (LBL). Acute lymphoblastic lymphoma (ALL)/LBL of the NK-lineage has not been defined, although such neoplasms should exist. Since T- and NK-lineages are so close in immature stages of differentiation that ALL/LBL of NK may have been sorted into T-lineage. The phenotypic records of the T-ALL/LBL in our laboratory indicated that CD7+CD5+CD2- is much higher in incidence than CD7+CD5-CD2+. This may reflect the size difference of physiological populations of T- and NK-lineage cells. Furthermore, the latter is of CD45RO type in contrast to the rest of the early-thymic (pro-thymic) T-ALL/LBL groups of CD45RA type. A CD56+ case of 4 CD7+CD5-CD2+ cases have been published as a case of LBL of NK-lineage. It is necessary to scrutinize CD7+CD5-CD2+ cells in order to clarify the phenotype of neoplastic and physiological NK cells in immature stages.
已知为自然杀伤（NK）细胞系的YT细胞，接受了爱泼斯坦-巴尔病毒（EBV）检测。简单培养的YT细胞（YT-O）以及两个不同的亚克隆，在用LMP-1探针进行的Southern印迹杂交中，显示出末端重复序列的连接DNA长度相同，这表明这三者在亚克隆之前就已经呈EBV阳性。YT-O表达有限量的EBNA2或LMP-1 mRNA，而在用EBNA2或LMP-1探针进行的Northern印迹分析中，两个亚克隆表达丰富的EBNA2或LMP-1 mRNA。因此，体外培养的细胞呈EBV阳性，因为YT-O中BL（伯基特淋巴瘤）型EBV基因表达一般不会发生在体外感染中。其余临床记录表明，该淋巴瘤为结外（血管中心性淋巴瘤），累及纵隔和肝脏，但不是淋巴结或淋巴母细胞淋巴瘤（LBL）。NK细胞系的急性淋巴细胞白血病（ALL）/LBL尚未明确界定，尽管这类肿瘤应该存在。由于T细胞系和NK细胞系在分化的不成熟阶段非常接近，以至于NK细胞系的ALL/LBL可能已被归类到T细胞系中。我们实验室中T-ALL/LBL的表型记录表明，CD7+CD5+CD2-的发生率远高于CD7+CD5-CD2+。这可能反映了T细胞系和NK细胞系细胞生理群体的大小差异。此外，与其余早期胸腺（前胸腺）T-ALL/LBL组的CD45RA型不同，后者为CD45RO型。在已发表的4例CD7+CD5-CD2+病例中，有1例CD56+病例被报道为NK细胞系LBL。有必要仔细研究CD7+CD5-CD2+细胞，以明确不成熟阶段肿瘤性和生理性NK细胞的表型。
