Vielvoye G J, Voogd J
J Comp Neurol. 1977 Sep 15;175(2):233-42. doi: 10.1002/cne.901750206.
Mossy fiber rosettes in the granular layer of the cerebellar cortex were studied after sections of the lateral funiculus of the spinal cord of the chicken and silver impregnation with the Fink-Heimer ('67) method I. After a survival time of two or three days two types of degenerated rosettes were found. The first type is characterized by digitiform protrusions, the second type of rosette is spherical. Both types are covered by small argyrophilic particles which disappear when the degeneration proceeds. With longer survival times the rosettes become swollen and finally disintegrate and lose their argyrophilia. After a survival time of 30 days only debris of rosettes can be found. These observations were used to determine the cerebellar cortical projection mossy fibers originating from segments of the spinal cord isolated in "successive degeneration" experiments consisting of a chronic cordotomy followed by an acute cordotomy rostral to the first one.
采用Fink-Heimer（1967年）I法对鸡脊髓外侧索进行切断并银浸染后，对小脑皮质颗粒层中的苔藓纤维玫瑰花结进行了研究。在存活两到三天后，发现了两种类型的变性玫瑰花结。第一种类型的特征是指状突起，第二种类型的玫瑰花结是球形的。两种类型都被小的嗜银颗粒覆盖，当变性进展时这些颗粒会消失。随着存活时间延长，玫瑰花结会肿胀，最终解体并失去嗜银性。在存活30天后，只能发现玫瑰花结的碎片。这些观察结果被用于确定在“连续变性”实验中分离出的脊髓节段所产生的小脑皮质投射苔藓纤维，该实验包括慢性脊髓切断术，随后在第一个切断部位的头侧进行急性脊髓切断术。
