Freedman S L, Voogd J, Vielvoye G J
J Comp Neurol. 1977 Sep 15;175(2):243-52. doi: 10.1002/cne.901750207.
This study identifies climbing fibers in the cerebellar cortex following brain stem lesions. Antegrade nerve fiber degeneration was studied after electrolytic destruction of rhombencephalic structures in 24 domestic fowl. Direct damage to the cerebellum was precluded by a retropharyngeal surgical approach. Only lesions that include the inferior olivary nucleus were compatible with Fink-Heimer staining of degeneratin nerve fibers in the Purkinje cell and molecular layer. Degenerating axons were identified as climbing fibers from their trajectory in association with Purkinje cell soma and dendrites. Terminal nerve endings were observed only in the basal one-third to one-half of the molecular layer. The presence of climbing fibers in only the contralateral molecular layer was observed in autoradiographic preparation subsequent to unilateral injection of tritiated leucine into the inferior olivary nucleus of three hens. The morphology of the labeled and impregnated degenerating nerve fibers closely coincides with information provided by previous investigators using Golgi stained histological preparations and electron microscopy.
本研究确定了脑干损伤后小脑皮质中的攀缘纤维。在对24只家禽的菱脑结构进行电解破坏后，研究了顺行性神经纤维变性。经咽后手术入路避免了对小脑的直接损伤。只有包括下橄榄核的损伤才与浦肯野细胞和分子层中变性神经纤维的芬克 - 海默染色相符。从与浦肯野细胞胞体和树突相关的轨迹来看，变性轴突被确定为攀缘纤维。仅在分子层底部三分之一至二分之一处观察到终末神经末梢。在三只母鸡的下橄榄核单侧注射氚标记亮氨酸后的放射自显影片中，仅在对侧分子层观察到攀缘纤维的存在。标记和浸染的变性神经纤维的形态与先前研究者使用高尔基染色组织学制剂和电子显微镜提供的信息密切相符。
