Ueda H, Fujimori O, Abe M
Department of Anatomy, Nagoya City University Medical School, Japan.
Arch Histol Cytol. 1996 Oct;59(4):389-97. doi: 10.1679/aohc.59.389.
An electron microscopic analysis with specific histochemical stainings for acidic glycoconjugates was carried out to examine the endothelium lining blood vessels of the rat spleen. Histochemical staining performed was the postembedding high or low iron diaminethiocarbohydrazide-silver protein-physical development (HID or LID-TCH-SP-PD) method, with or without prior digestion with acidic glycoconjugate-degrading enzymes, such as heparitinase, testicular hyaluronidase, chondroitinase B and neuraminidase. The results indicated that the acidic glycoconjugates in the basal lamina of the endothelial cells lining the four types of blood vessel (central arteries, arterial capillaries, splenic sinuses and pulp veins) were heparan sulfate, chondroitin sulfate A and/or C, chondroitin sulfate B and sialic acid residues. In the endothelial cells lining the central arteries, arterial capillaries and pulp veins, the surface coat of the luminal plasma membrane included heparan sulfate, chondroitin sulfate A and/or C, chondroitin sulfate B and sialic acid residues, whereas the corresponding ultrastructure of the splenic sinuses was devoid of detectable amounts of acidic glycoconjugates. This suggests that such characteristic histochemical features of the endothelium in the four types of the splenic blood vessel can be related to the possible physiological functions of the spleen.
采用酸性糖缀合物特异性组织化学染色进行电子显微镜分析，以检查大鼠脾脏血管的内皮。所进行的组织化学染色是包埋后高铁或低铁二氨基硫代碳酰肼 - 银蛋白 - 物理显影（HID或LID - TCH - SP - PD）方法，在有或没有用酸性糖缀合物降解酶（如乙酰肝素酶、睾丸透明质酸酶、软骨素酶B和神经氨酸酶）预先消化的情况下进行。结果表明，四种类型血管（中央动脉、动脉毛细血管、脾窦和髓静脉）内衬内皮细胞的基膜中的酸性糖缀合物是硫酸乙酰肝素、硫酸软骨素A和/或C、硫酸软骨素B和唾液酸残基。在中央动脉、动脉毛细血管和髓静脉内衬的内皮细胞中，腔面膜的表面涂层包含硫酸乙酰肝素、硫酸软骨素A和/或C、硫酸软骨素B和唾液酸残基，而脾窦的相应超微结构未检测到酸性糖缀合物。这表明脾脏四种类型血管内皮的这种特征性组织化学特征可能与脾脏的潜在生理功能有关。
