Hegge F N, Mahler D J, Larson S M
J Nucl Med. 1977 Sep;18(9):937-9.
Twenty-four hours after the administration of Ga-67 citrate and Fe-59 citrate, rabbits were killed and their livers removed and homogenized. Labile proteins in the filtered liver homogenates were denatured; ferritin was then crystallized from the supernatants by cadmium sulfate. Sephadex G-200 gel filtration of the ferritin fractions was done to determine the distribution of molecular weights in the substances associated with Ga-67 and Fe-59. It was found that Ga-67 was incorporated into the crystallizable ferritin fraction of rabbit hepatocytes with approximately one-sixth the uptake of simultaneously administered Fe-59. Gel-filtration chromatography confirmed that both the Ga-67 and the Fe-59 of the crystallizable ferritin fraction were associated with substances of the appropriate molecular weight for ferritin.
在给予柠檬酸镓-67和柠檬酸铁-59 24小时后，处死兔子并取出肝脏进行匀浆。对过滤后的肝脏匀浆中的不稳定蛋白质进行变性处理；然后通过硫酸镉从上清液中使铁蛋白结晶。对铁蛋白级分进行葡聚糖凝胶G-200凝胶过滤，以确定与镓-67和铁-59相关物质的分子量分布。结果发现，镓-67以约为同时给予的铁-59摄取量六分之一的比例掺入兔肝细胞的可结晶铁蛋白级分中。凝胶过滤色谱法证实，可结晶铁蛋白级分中的镓-67和铁-59均与铁蛋白分子量合适的物质相关联。
