Nascimento D C, Sakate M
Department of Pharmacology, São Paulo State University (UNESP), Botucatu, Brazil.
Pharmacol Res. 1996 Apr-May;33(4-5):251-4. doi: 10.1006/phrs.1996.0035.
The action of hyperosmotic nutrient solutions on the depressive effects of carbofuran was studied on spontaneously contracting guinea pig atria. The force and frequency of contraction were recorded using an isotonic lever. Carbofuran 33.0 micrograms ml-1 produced a gradual depressive effect. The time for stabilization of the depressive effect was 6.0 +/- 2.3 min. After the depressive effect of carbofuran was established, NaCl, mannitol, or urea were introduced into the organ bath to increase the osmolarity of the nutrient solution to about 100 mosmol kg-1 of water. This resulted in a reversal of the contraction force and frequency to the initial levels. The addition of the osmotic agents at the same concentrations before the addition of 33.0 micrograms ml-1 carbofuran avoided its depressive effect on guinea pig atria.
在自发性收缩的豚鼠心房上，研究了高渗营养液对呋喃丹抑制作用的影响。使用等张杠杆记录收缩力和频率。33.0微克/毫升的呋喃丹产生逐渐的抑制作用。抑制作用稳定的时间为6.0±2.3分钟。在呋喃丹的抑制作用确立后，将氯化钠、甘露醇或尿素引入器官浴中，使营养液的渗透压增加到约100毫摩尔/千克水。这导致收缩力和频率恢复到初始水平。在加入33.0微克/毫升呋喃丹之前，加入相同浓度的渗透剂可避免其对豚鼠心房的抑制作用。
