Takahashi M, Yamada T, Tooyama I, Moroo I, Kimura H, Yamamoto T, Okada H
Choju Medical Institute, Noyori Fukushi-mura Hospital, Toyohashi-shi, Aichi-keu, Japan.
Neurosci Lett. 1996 Feb 9;204(3):201-4. doi: 10.1016/0304-3940(96)12357-0.
Neurotrophic effects resulting from the insulin/insulin receptor system have been recognized as important in determining the etiological basis of neurodegenerative disorders. In Parkinson's disease, selective neuronal loss in the substantia nigra is accompanied by decreased immunoreactivity of the insulin receptor as determined using immunohistochemical studies. We performed semiquantitative mRNA analysis by reverse transcription-polymerase chain reaction (RT-PCR) using specific primers for human insulin receptor exon 22, which encodes a region of the beta subunit of the receptor serving as a tyrosine kinase domain. The relative levels of mRNA in the substantia nigra from Parkinson's brain tissues showed a marked depression compared with those of normal controls. Further investigations are needed to decide whether this is a primary, disease-specific alteration of gene expression or merely a secondary process.
胰岛素/胰岛素受体系统产生的神经营养作用在确定神经退行性疾病的病因基础方面已被认为很重要。在帕金森病中，黑质中的选择性神经元丢失伴随着胰岛素受体免疫反应性的降低，这是通过免疫组织化学研究确定的。我们使用针对人胰岛素受体外显子22的特异性引物，通过逆转录-聚合酶链反应（RT-PCR）进行了半定量mRNA分析，该外显子编码受体β亚基的一个区域，该区域作为酪氨酸激酶结构域。与正常对照组相比，帕金森病脑组织黑质中mRNA的相对水平显著降低。需要进一步研究来确定这是基因表达的原发性、疾病特异性改变还是仅仅是一个继发性过程。
