Hsieh J T, Chien C T, Liu S P, Chen C F, Lai M K
Department of Urology, National Taiwan University Hospital, Taipei, Taiwan.
Neurosci Lett. 1996 Feb 9;204(3):215-7. doi: 10.1016/0304-3940(96)12354-5.
This investigation demonstrated a good model to record the pressure change of rat seminal vesicle (SV) in response to electrical stimulation (ES) of lesser splanchnic nerve (LSN) in vivo. LSN was easily identified and isolated grossly. Antegrade catheterization via the SV tail to the main lumen was performed similar to the technique of i.v. catheterization, and the pressure recording was quite satisfactory. The vesicle response to ES was frequency-dependent and reproducible. Under the conditions of 10 V and 1 ms, the maximal vesicle pressure was 81.7 +/- 2.8 mmHg with 80 Hz of ES. The inhibitory response of SV to ES by clomipramine was concentration-dependent. Clomipramine (3 mg/kg) could inhibit 53% of control responses (n = 7). This experimental model is relevant to neurophysiologic and neuropharmacologic studies of SV.
本研究证明了一种在体内记录大鼠精囊（SV）对内脏小神经（LSN）电刺激（ES）反应时压力变化的良好模型。LSN在大体上易于识别和分离。通过SV尾部向主腔进行顺行插管，其操作类似于静脉插管技术，压力记录效果相当令人满意。精囊对ES的反应具有频率依赖性且可重复。在10 V和1 ms的条件下，80 Hz的ES刺激时精囊最大压力为81.7±2.8 mmHg。氯米帕明对精囊ES反应的抑制作用呈浓度依赖性。氯米帕明（3 mg/kg）可抑制53%的对照反应（n = 7）。该实验模型与精囊的神经生理学和神经药理学研究相关。
