De Brabander H F, Batjoens P, Courtheyn D, Vercammen J, De Wasch K
Department of Veterinary Food Inspection, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
J Chromatogr A. 1996 Oct 25;750(1-2):105-14. doi: 10.1016/0021-9673(96)00411-6.
Chromatographic techniques such as GC-MS play a most important role in modern multi-residue analysis of anabolic steroids. The major difference between GC-MS apparatus from different manufacturers is the way of detection and recording. Most apparatus use selected-ion monitoring (SIM) for the determination of low concentrations. Systems based on ion trap technology record in full-scan to even picogram concentrations using a computer algorithm to compare the most important peaks of the mass spectrum of the unknown to those of the standard. In this investigation the possibilities of ion trap GC-MS and the recently released GCQ MS and MS2 for the analysis of anabolics in biological material are compared.
诸如气相色谱 - 质谱联用（GC-MS）等色谱技术在合成代谢类固醇的现代多残留分析中起着至关重要的作用。不同制造商生产的GC-MS仪器之间的主要区别在于检测和记录方式。大多数仪器使用选择离子监测（SIM）来测定低浓度物质。基于离子阱技术的系统通过计算机算法将未知物质谱图中最重要的峰与标准物的峰进行比较，甚至可以全扫描记录到皮克级浓度。在本研究中，对离子阱GC-MS以及最近推出的GCQ MS和MS2用于分析生物材料中合成代谢物的可能性进行了比较。
