van Stijn F, Kerkhoff M A, Vandeginste B G
Unilever Research Laboratorium, Vlaardingen, Netherlands.
J Chromatogr A. 1996 Oct 25;750(1-2):263-73. doi: 10.1016/0021-9673(96)00284-1.
Various off-line methods for clean-up and sample enrichment are available for the analysis of polycyclic aromatic hydrocarbons (PAHs) in edible oils and fats. These methods consist of laborious and time consuming procedures. This study reports an on-line method using LC-LC coupling. After clean-up of the sample on a donor-acceptor complex chromatography (DACC) column the PAHs are transferred to and separated on an analytical HPLC column. Quantification is carried out with fluorescence detection. The DACC column clean-up is fast and is carried out during the HPLC run of the previous sample. Compared to the traditional methods this automated on-line method saves considerable time and significantly reduces the amount of solvent waste. The method uses common HPLC equipment and its performance has been evaluated.
有多种离线净化和样品富集方法可用于分析食用油脂中的多环芳烃（PAHs）。这些方法包括费力且耗时的程序。本研究报告了一种使用液相色谱-液相色谱联用的在线方法。在供体-受体络合色谱（DACC）柱上对样品进行净化后，PAHs被转移至分析型高效液相色谱柱上并进行分离。采用荧光检测进行定量分析。DACC柱净化速度快，且在前一个样品的高效液相色谱运行期间即可完成。与传统方法相比，这种自动化在线方法节省了大量时间，并显著减少了溶剂浪费量。该方法使用常见的高效液相色谱设备，且已对其性能进行了评估。
