Ohsawa N, Sakamoto M, Saito T, Kobayashi M, Hirohashi S
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
J Hepatol. 1996 Nov;25(5):655-62. doi: 10.1016/s0168-8278(96)80235-2.
AIMS/METHODS: Numerical aberrations of chromosomes 7,8,9,10,12,17,18, X and Y were examined in 38 hepatocellular carcinoma specimens using centromere-specific DNA probes by the fluorescence in situ hybridization method.
Numerical aberrations in more than one of the chromosomes examined were found in 27 of 38 (71%) specimens; 6 of 15 (40%) well-differentiated hepatocellular carcinomas; 15 of 17 (88%) moderately differentiated hepatocellular carcinomas; and all of 6 (100%) poorly differentiated hepatocellular carcinomas. Of 6 early hepatocellular carcinomas, numerical chromosome aberrations were detected in 2. The incidence of numerical chromosome aberrations was 93.8% in patients with portal vein thromboses and/or intrahepatic metastases, 52.4% without portal vein thromboses and/or intrahepatic metastases (p < 0.05), while 89.5% of patients with a tumor more than 3 cm in diameter and 50.0% with a tumor less than 3 cm in diameter had chromosome aberrations (p < 0.05).
These results suggest that numerical chromosome aberrations start to occur in the early stage of hepatocellular carcinoma and to accumulate during tumor progression.
目的/方法：采用荧光原位杂交法，使用着丝粒特异性DNA探针，检测38例肝细胞癌标本中7、8、9、10、12、17、18、X和Y染色体的数目畸变。
在38例标本中的27例（71%）检测到不止一条被检测染色体存在数目畸变；在15例高分化肝细胞癌中有6例（40%）；在17例中分化肝细胞癌中有15例（88%）；在6例低分化肝细胞癌中全部（100%）存在。在6例早期肝细胞癌中，2例检测到染色体数目畸变。门静脉血栓形成和/或肝内转移患者的染色体数目畸变发生率为93.8%，无门静脉血栓形成和/或肝内转移患者的发生率为52.4%（p<0.05），而直径大于3 cm的肿瘤患者中89.5%存在染色体畸变，直径小于3 cm的肿瘤患者中50.0%存在染色体畸变（p<0.05）。
这些结果表明，染色体数目畸变在肝细胞癌早期开始出现，并在肿瘤进展过程中累积。
