Nagase S, Aoyagi K, Hirayama A, Gotoh M, Ueda A, Tomida C, Kikuchi H, Takemura K, Koyama A
Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Japan.
Nephron. 1996;74(3):555-60. doi: 10.1159/000189451.
This study aims to raise the possibility of methylguanidine, a peroxidative product of creatinine, as a measure of the peroxidative state. As a known standard, we measured the inhibitory effect of uremic serum on the NADPH-dependent microsomal lipid peroxidation. This is an established method for evaluating the peroxidative state and is compared to the effect of uremic serum on methylguanidine synthesis. The study shows decreased serum antioxidant activity in hemodialysis patients by both methods, though there is no correlation between them. These results support the use of methylguanidine as a peroxidative marker and suggest a difference in the reactive oxygen species involved in the reactions of methylguanidine synthesis and microsomal lipid peroxidation.
本研究旨在提高肌酐的过氧化产物甲基胍作为过氧化状态指标的可能性。作为已知标准，我们测定了尿毒症血清对NADPH依赖性微粒体脂质过氧化的抑制作用。这是一种评估过氧化状态的既定方法，并与尿毒症血清对甲基胍合成的影响进行比较。研究表明，通过这两种方法均发现血液透析患者血清抗氧化活性降低，尽管它们之间没有相关性。这些结果支持将甲基胍用作过氧化标志物，并表明参与甲基胍合成反应和微粒体脂质过氧化反应的活性氧存在差异。
