睡眠-觉醒周期中外侧膝状核内中脑桥脑PGO发放阳性细胞的乙酰胆碱释放及5-羟色胺能调节

Acetylcholine releases of mesopontine PGO-on cells in the lateral geniculate nucleus in sleep-waking cycle and serotonergic regulation.

Kodama T, Honda Y

Department of Psychology, Tokyo Metropolitan Institute for Neuroscience, Japan.

Prog Neuropsychopharmacol Biol Psychiatry. 1996 Oct;20(7):1213-27. doi: 10.1016/s0278-5846(96)00107-8.

Abstract

Five adult cats were prepared with standard sleep-recording electrodes, microinjection cannulae in the bilateral mesopontine tegmentum X area and cannulae for microdialysis probes in the LGN. 2. Dialysates were collected at 5 min intervals during SWS, REM sleep and wakefulness, and during pre- and post-stimulation periods of 5MeODMT microinjection in the bilateral mesopontine tegmentum of freely moving cats. 3. A REM sleep-specific increase of ACh release was observed in the LGN, but not out of the LGN. 4. ACh release was depressed up to 33% by bilateral 5MeODMT microinjection in the mesopontine tegmentum where the microinjection of carbachol also depressed ACh in the LGN probably by stimulating the auto receptor. 5. Our observations indicate that PGO-on neurons of mesopontine tegmentum release more ACh in the LGN during REM sleep by burst discharge than during wakefulness and SWS, and that presumptive cholinergic PGO-on cells are regulated by serotonergic inputs.

摘要

对5只成年猫进行准备，在其双侧脑桥中脑被盖X区植入标准睡眠记录电极、微量注射套管，并在外侧膝状体植入用于微透析探针的套管。2. 在慢波睡眠、快速眼动睡眠和清醒期间，以及在自由活动的猫双侧脑桥中脑被盖进行5-甲氧基-N,N-二甲基色胺（5MeODMT）微量注射的刺激前和刺激后期间，每隔5分钟收集一次透析液。3. 观察到外侧膝状体中乙酰胆碱（ACh）释放有快速眼动睡眠特异性增加，但外侧膝状体之外未观察到。4. 在脑桥中脑被盖进行双侧5MeODMT微量注射使ACh释放降低达33%，在该处注射卡巴胆碱可能通过刺激自身受体也降低了外侧膝状体中的ACh。5. 我们的观察结果表明，脑桥中脑被盖的脑桥-膝状体-枕叶锋电位（PGO）开启神经元在快速眼动睡眠期间通过爆发式放电比在清醒和慢波睡眠期间在外侧膝状体释放更多的ACh，并且推测胆碱能PGO开启细胞受5-羟色胺能输入调节。