Calvo J M, Datta S, Quattrochi J, Hobson J A
Laboratory of Neurophysiology, Harvard Medical School, Boston, MA 02115.
Arch Ital Biol. 1992 Oct;130(4):285-301.
The hypothesis that REM sleep is cholinergically mediated is supported by the identification of a cholinoceptive trigger zone in the FTG. Since this trigger zone is devoid of cholinergic neurons, the aim of the present study was to test the hypothesis that a cholinergic drive for REM sleep may come from the cholinergic cells of the PBL region. Chronically implanted freely moving cats with electrodes for sleep and PGO wave recordings were used. Guide tubes were implanted for carbachol microinjections (4 micrograms/250 nl) in the PBL and FTG. All microinjections were delivered in close vicinity of ChAT+ cholinergic cells in the PBL region. Results showed that a single unilateral carbachol microinjection into the PBL induced sustained (24 hr) state-independent ipsilateral PGO wave activity. This PGO wave activity was followed by a prolonged enhancement of REM sleep lasting for more than six days. We also observed that REM enhancement was followed by a delayed but marked enhancement of S sleep episodes with PGO waves (SP), which are normally brief transitions from S to REM sleep. Our findings strongly support the hypothesis that cholinergic drive for REM sleep comes from the lateral pontine tegmentum and we suggest that the PBL region plays a major role in both PGO wave generation and long-term regulation of REM sleep induction.
快速眼动睡眠由胆碱能介导这一假说，得到了在延髓巨细胞网状核中发现胆碱能感受触发区的支持。由于该触发区不含胆碱能神经元，本研究的目的是检验快速眼动睡眠的胆碱能驱动可能来自脑桥被盖外侧区胆碱能细胞这一假说。使用长期植入电极用于睡眠和脑桥-膝状体-枕叶锋电位（PGO波）记录的自由活动猫。植入导管用于在脑桥被盖外侧区和延髓巨细胞网状核中微量注射卡巴胆碱（4微克/250纳升）。所有微量注射均在脑桥被盖外侧区胆碱乙酰转移酶阳性（ChAT+）胆碱能细胞附近进行。结果表明，向脑桥被盖外侧区单次单侧微量注射卡巴胆碱可诱导持续（24小时）的与状态无关的同侧PGO波活动。这种PGO波活动之后是快速眼动睡眠的延长增强，持续超过六天。我们还观察到，快速眼动睡眠增强之后是伴有PGO波的慢波睡眠发作（SP）延迟但显著增强，SP通常是从慢波睡眠到快速眼动睡眠的短暂过渡。我们的研究结果有力地支持了快速眼动睡眠的胆碱能驱动来自脑桥外侧被盖区这一假说，并且我们认为脑桥被盖外侧区在PGO波产生和快速眼动睡眠诱导的长期调节中均起主要作用。
