犬皮肤厚皮马拉色菌定量培养的培养基及培养条件比较

Comparison of media and conditions of incubation for the quantitative culture of Malassezia pachydermatis from canine skin.

作者信息

Bond R, Lloyd D H

机构信息

Department of Small Animal Medicine and Surgery, Royal Veterinary College, University of London, Hatfield, Herts.

出版信息

Res Vet Sci. 1996 Nov;61(3):273-4. doi: 10.1016/s0034-5288(96)90078-8.

DOI:10.1016/s0034-5288(96)90078-8

PMID:8938862

Abstract

Fifty-five swab-wash specimens from dogs were cultured at 32 degrees C on Sabouraud's dextrose agar either with or without 1 per cent Tween 80. Ushijima's medium A and modified Dixon's and Leeming's media. The counts of Malassezia pachydermatis were not significantly different after three or seven days of incubation. Colony counts on contact plates were significantly greater after incubation for seven days on Sabouraud's dextrose (P < 0.001) and modified Dixon's agars (P < 0.05) than after three days at 26 degrees C, but not at 32 degrees C. Counts on Sabouraud's or modified Dixon's agars after incubation at 32 degrees C and 37 degrees C were not significantly different. When compared with aerobic culture, an atmosphere containing 5 to 10 per cent carbon dioxide significantly (P < 0.01) increased the frequency of isolation and colony counts on Sabouraud's dextrose agar but not on modified Dixon's agar.

摘要

从犬类采集的55份拭子洗涤标本在32摄氏度下，于添加或不添加1%吐温80的沙氏葡萄糖琼脂、Ushijima培养基A以及改良的Dixon培养基和Leeming培养基上进行培养。在孵育三天或七天后，厚皮马拉色菌的计数没有显著差异。在26摄氏度下，接触平板上的菌落计数在孵育七天后于沙氏葡萄糖琼脂（P < 0.001）和改良的Dixon琼脂（P < 0.05）上显著高于孵育三天后，但在32摄氏度下并非如此。在32摄氏度和37摄氏度下孵育后，沙氏葡萄糖琼脂或改良的Dixon琼脂上的计数没有显著差异。与需氧培养相比，含有5%至10%二氧化碳的环境显著（P < 0.01）提高了沙氏葡萄糖琼脂上的分离频率和菌落计数，但在改良的Dixon琼脂上并非如此。