Grupo de Investigación Celular y Molecular de Microorganismos Patógenos (CeMoP), Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
Unidad de Investigación en Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Front Cell Infect Microbiol. 2020 May 5;10:199. doi: 10.3389/fcimb.2020.00199. eCollection 2020.
and are lipophilic and lipid dependent yeasts, associated with the skin microbiota in humans and domestic animals, respectively. Although they are commensals, under specific conditions they become pathogens, causing skin conditions, such as pityriasis versicolor, dandruff/seborrheic dermatitis, folliculitis in humans, and dermatitis and otitis in dogs. Additionally, these species are associated with fungemia in immunocompromised patients and low-weight neonates in intensive care units with intravenous catheters or with parenteral nutrition and that are under-treatment of broad-spectrum antibiotics. The host-pathogen interaction mechanism in these yeasts is still unclear; for this reason, it is necessary to implement suitable new host systems, such as . This infection model has been widely used to assess virulence, host-pathogen interaction, and antimicrobial activity in bacteria and fungi. Some advantages of the model are: (1) the immune response has phagocytic cells and antimicrobial peptides that are similar to those in the innate immune response of human beings; (2) no ethical implications; (3) low cost; and (4) easy to handle and inoculate. This study aims to establish as an infection model for and . To achieve this objective, first, larvae were first inoculated with different inoculum concentrations of these two species, 1.5 × 10 CFU/mL, 1.5 × 10 CFU/mL, 1.5 × 10 CFU/mL, and 11.5 × 10 CFU/mL, and incubated at 33 and 37°C. Then, for 15 days, the mortality and melanization were evaluated daily. Finally, the characterization of hemocytes and fungal burden assessment were as carried out. It was found that at 33 and 37°C both and successfully established a systemic infection in . proved to be slightly more virulent than at a temperature of 37°C. The results suggest that larvae mortality and melanization is dependent on the specie of , the inoculum concentration and the temperature. According to the findings, can be used as an model of infection to conduct easy and reliable approaches to boost our knowledge of the genus.
和 是亲脂性和脂质依赖性酵母，分别与人类和家畜的皮肤微生物群有关。虽然它们是共生菌，但在特定条件下它们会成为病原体，导致皮肤状况，如花斑癣、头皮屑/脂溢性皮炎、人类的滤泡炎，以及狗的皮炎和耳炎。此外，这些物种与免疫功能低下患者的真菌血症、重症监护病房中体重较轻的新生儿、静脉导管或肠外营养以及广谱抗生素治疗不当有关。这些酵母中宿主-病原体相互作用的机制尚不清楚；因此，有必要实施合适的新宿主系统，如 。这种感染模型已广泛用于评估细菌和真菌的毒力、宿主-病原体相互作用和抗菌活性。 模型的一些优点是：（1）免疫反应具有吞噬细胞和抗菌肽，类似于人类先天免疫反应中的吞噬细胞和抗菌肽；（2）无伦理问题；（3）成本低；（4）易于处理和接种。本研究旨在建立 作为 和 的 感染模型。为了实现这一目标，首先，将幼虫分别接种不同浓度的这两种 种，1.5×10 CFU/mL、1.5×10 CFU/mL、1.5×10 CFU/mL 和 11.5×10 CFU/mL，在 33 和 37°C 下孵育。然后，在 15 天内每天评估死亡率和黑化。最后，进行血细胞特征分析和真菌载量评估。结果发现，在 33 和 37°C 下， 和 都成功地在 中建立了系统性感染。 比 37°C 时的 略具有毒力。结果表明，幼虫死亡率和黑化取决于 的种、接种浓度和温度。根据这些发现，幼虫可作为 感染模型，用于进行简便可靠的方法，以增进我们对 属的了解。
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