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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Departamento de Ciencias Biológicas, Universidad Nacional de La Plata, Argentina.

Comp Biochem Physiol B Biochem Mol Biol. 1996 Oct;115(2):175-82. doi: 10.1016/0305-0491(96)00079-x.

S-type lectin from Bufo arenarum embryos at blastula stage was purified by affinity chromatography. The molecule is a dimer with equal-sized monomers and the apparent subunit molecular weight was found to be 14.5 kDa. 2. Analytical isoelectric focusing of the pure lectin showed an acidic pI of 4.7. 3. Inhibition of the hemagglutination by mono- and oligosaccharides revealed a specificity for sugars bearing a beta-galactoside configuration. 4. Crossreactivity studies between the blastula lectin and the one purified earlier from adult ovary performed by immunodotting, ELISA and immunoblotting showed that these lectins share many epitopes.

通过亲和层析法纯化了来自囊胚期阿根廷蟾蜍胚胎的S型凝集素。该分子是由等大小单体组成的二聚体，其亚基表观分子量为14.5 kDa。2. 对纯凝集素进行的分析等电聚焦显示其酸性pI为4.7。3. 单糖和寡糖对血凝反应的抑制作用表明该凝集素对具有β-半乳糖苷构型的糖具有特异性。4. 通过免疫斑点法、酶联免疫吸附测定法和免疫印迹法对囊胚凝集素与先前从成年卵巢中纯化的凝集素进行的交叉反应性研究表明，这些凝集素具有许多共同表位。

Departamento de Ciencias Biológicas, Universidad Nacional de La Plata, Argentina.

Comp Biochem Physiol B Biochem Mol Biol. 1996 Oct;115(2):175-82. doi: 10.1016/0305-0491(96)00079-x.

S-type lectin from Bufo arenarum embryos at blastula stage was purified by affinity chromatography. The molecule is a dimer with equal-sized monomers and the apparent subunit molecular weight was found to be 14.5 kDa. 2. Analytical isoelectric focusing of the pure lectin showed an acidic pI of 4.7. 3. Inhibition of the hemagglutination by mono- and oligosaccharides revealed a specificity for sugars bearing a beta-galactoside configuration. 4. Crossreactivity studies between the blastula lectin and the one purified earlier from adult ovary performed by immunodotting, ELISA and immunoblotting showed that these lectins share many epitopes.

通过亲和层析法纯化了来自囊胚期阿根廷蟾蜍胚胎的S型凝集素。该分子是由等大小单体组成的二聚体，其亚基表观分子量为14.5 kDa。2. 对纯凝集素进行的分析等电聚焦显示其酸性pI为4.7。3. 单糖和寡糖对血凝反应的抑制作用表明该凝集素对具有β-半乳糖苷构型的糖具有特异性。4. 通过免疫斑点法、酶联免疫吸附测定法和免疫印迹法对囊胚凝集素与先前从成年卵巢中纯化的凝集素进行的交叉反应性研究表明，这些凝集素具有许多共同表位。