Elola M T, Fink N E
Departamento de Ciencias Biológicas, Universidad Nacional de La Plata, Argentina.
Biol Res. 1997;30(3):99-104.
Galectins (S-type or S-Lac lectins) are a well-defined family of beta-galactoside animal lectins characterized by a high sequence homology in the carbohydrate-binding domain. We have previously purified and characterized the S-type lectin from the ovary of the toad Bufo arenarum. In this study, we purified the S-type lectins from Bufo arenarum ovary and human spleen by an improved method which included ion exchange and affinity chromatography. Antibody cross-reactivities between both lectins and some other S-type lectins showed that they share epitopes. Glycosylation studies carried out with detection/differentiation kits suggested that both lectins are not glycosylated.
半乳糖凝集素（S型或S-Lac凝集素）是一类明确的β-半乳糖苷动物凝集素家族，其特征在于碳水化合物结合结构域具有高度的序列同源性。我们之前已从蟾蜍Bufo arenarum的卵巢中纯化并鉴定了S型凝集素。在本研究中，我们通过一种改进的方法从Bufo arenarum卵巢和人脾脏中纯化了S型凝集素，该方法包括离子交换和亲和色谱法。两种凝集素与其他一些S型凝集素之间的抗体交叉反应性表明它们共享表位。使用检测/鉴别试剂盒进行的糖基化研究表明这两种凝集素都没有糖基化。
