Counsell C M, Bond J F, Ohman J L, Greenstein J L, Garman R D
ImmuLogic Pharmaceutical Corporation, Waltham, MA 02154, USA.
J Allergy Clin Immunol. 1996 Nov;98(5 Pt 1):884-94. doi: 10.1016/s0091-6749(96)80004-2.
A heterodimeric acidic glycoprotein (Fel d 1) has been defined as the major allergen of the domestic cat. Because T-cell help is required for the initiation and maintenance of allergic responses, it is of importance to determine the T-cell-reactive regions of the Fel d 1 molecule.
Overlapping peptides corresponding to the two chains of Fel d 1 were tested in proliferation assays on polyclonal T-cell lines and for the ability to bind Fel d 1-specific IgE in ELISA and histamine release assays.
Assay of T-cell lines derived from 53 subjects allergic to cats demonstrated that the majority of T-cell reactivity is found in chain 1 of Fel d 1. Two peptides (Fel-1 and Fel-2) containing major epitopes, alone or as a mixture, efficiently activated T cells and exhibited minimal detectable reactivity with IgE by ELISA or histamine release assay.
Two Fel d 1 peptides containing major T-cell epitopes have been identified, have been shown to bind minimal Fel d 1-specific IgE, and are now being tested for the ability to decrease T-cell responses in patients with cat allergy as a new form of immunotherapy.
一种异二聚体酸性糖蛋白（猫过敏原1）已被确定为家猫的主要过敏原。由于过敏反应的启动和维持需要T细胞辅助，因此确定猫过敏原1分子的T细胞反应区域非常重要。
在多克隆T细胞系的增殖试验中测试了与猫过敏原1两条链相对应的重叠肽，并在ELISA和组胺释放试验中测试了其结合猫过敏原1特异性IgE的能力。
对53名对猫过敏的受试者来源的T细胞系进行检测，结果表明，大多数T细胞反应性存在于猫过敏原1的链1中。两种含有主要表位的肽（Fel-1和Fel-2）单独或混合使用时，能有效激活T细胞，并且通过ELISA或组胺释放试验检测，它们与IgE的反应性极低。
已鉴定出两种含有主要T细胞表位的猫过敏原1肽，它们与猫过敏原1特异性IgE的结合极少，目前正在作为一种新的免疫疗法形式，测试其降低猫过敏患者T细胞反应的能力。
