Lazaro J B, Kitzmann M, Cavadore J C, Muller Y, Clos J, Fernandez A, Lamb N J
Cell Biology Unit, Université Montpellier II, France.
Neurosci Lett. 1996 Oct 25;218(1):21-4. doi: 10.1016/0304-3940(96)13106-2.
We have examined the expression of cyclin dependent kinase (cdk) 5 protein kinase and p35nck5a, its activator subunit, during postnatal neurogenesis in rat cerebellum, using mono-specific antibodies. Both cdk5 and p35nck5a are present and associated in proliferative stages, although cdk5-p35 kinase activity is barely detectable. Cdk5-p35 activity, but not the expression of either subunit, increases up to 6-fold during neuronal differentiation. Since we observe that cdk5 is phosphorylated on tyrosine in proliferative, but not in post-mitotic stages, we suggest that post-translational regulatory mechanisms control cdk5-p35 protein kinase activity during neurogenesis.
我们使用单特异性抗体，研究了细胞周期蛋白依赖性激酶（cdk）5蛋白激酶及其激活亚基p35nck5a在大鼠小脑出生后神经发生过程中的表达。cdk5和p35nck5a在增殖阶段均存在且相关联，尽管cdk5 - p35激酶活性几乎检测不到。在神经元分化过程中，cdk5 - p35活性增加至6倍，但任一亚基的表达均未增加。由于我们观察到cdk5在增殖阶段而非有丝分裂后阶段的酪氨酸位点发生磷酸化，我们认为翻译后调控机制在神经发生过程中控制着cdk5 - p35蛋白激酶的活性。
