McNabb D S, Guarente L
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Curr Opin Biotechnol. 1996 Oct;7(5):554-9. doi: 10.1016/s0958-1669(96)80061-9.
The two-hybrid system is a powerful approach for examining protein-protein interactions. Recently, the utility of the system has been extended to include the genome-wide mapping of protein-protein interactions and the identification of peptide inhibitors of protein interactions. In addition, immunophilins and their chemical ligands are providing useful reagents for generating conditional protein-protein interactions in vivo to dissect intracellular signaling pathways.
双杂交系统是研究蛋白质-蛋白质相互作用的一种强大方法。最近，该系统的应用范围已扩展到包括蛋白质-蛋白质相互作用的全基因组图谱绘制以及蛋白质相互作用肽抑制剂的鉴定。此外，免疫亲和素及其化学配体为在体内产生条件性蛋白质-蛋白质相互作用以剖析细胞内信号通路提供了有用的试剂。
