Weickert M J, Doherty D H, Best E A, Olins P O
Somatogen Inc., 2545 Central Avenue, Boulder, CO 80301, USA.
Curr Opin Biotechnol. 1996 Oct;7(5):494-9. doi: 10.1016/s0958-1669(96)80051-6.
Escherichia coli has long been the primary prokaryotic host for the synthesis of heterologous proteins. Recent advances have been made in the expression of complex proteins as soluble, functional molecules, complete with prosthetic groups, disulfide bonds, and quaternary structure. The development of alternative promoter and induction strategies has improved the options available for manipulating the expression conditions, which are frequently critical to soluble yield.
长期以来，大肠杆菌一直是合成异源蛋白的主要原核宿主。在将复杂蛋白表达为可溶性功能分子方面取得了新进展，这些分子具有辅基、二硫键和四级结构。替代启动子和诱导策略的发展增加了可用于调控表达条件的选择，而表达条件往往对可溶性产量至关重要。
