Zareba T W, Pascu C, Hryniewicz W, Wadström T
Sera and Vaccines Central Research Laboratory, Chelmska str 30/34, PL 00-725 Warsaw, Poland.
Curr Microbiol. 1997 Jan;34(1):6-11. doi: 10.1007/s002849900135.
Forty-four enterococcal strains isolated from human clinical specimens were investigated for binding of 125I-labeled fibronectin, vitronectin, thrombospondin, lactoferrin, and collagen type I and IV, and for cell surface hydrophobicity. Most strains expressed low binding of iodine-labeled human fibronectin, collagen I and IV, and higher binding of human vitronectin, human lactoferrin, and human thrombospondin. Bacteria grown in Todd-Hewitt broth exhibited increased binding to vitronectin and thrombospondin. In particle agglutination assays (PAA), Enterococcus faecalis strains reacted strongly with coated latex beads in contrast to E. faecium strains, which generally did not react. The ability of enterococci to bind ECM proteins was affected by heating and proteolytic digestion, suggesting that some protein-binding components become surface exposed after treatment with proteases. The binding of 125I-labeled proteins to E. faecalis strain E70 was inhibited when cells were preincubated with unlabeled proteins. Preincubating cells with sulfated polymers such as dextran sulfate (Mr 5000 and 8000), pentosan sulfate and heparin decreased binding of vitronectin, lactoferrin, and thrombospondin. The binding of lactoferrin and thrombospondin was also decreased when bacteria were preincubated with galactose, fucose, and mannosamine, but not with mannose. All of 30 E. faecalis strains expressed pronounced surface hydrophobicity, but 10 of 14 E. faecium strains showed hydrophilic cell surface.
对从人类临床标本中分离出的44株肠球菌进行了研究，检测其对125I标记的纤连蛋白、玻连蛋白、血小板反应蛋白、乳铁蛋白以及I型和IV型胶原的结合情况，同时检测细胞表面疏水性。大多数菌株对碘标记的人纤连蛋白、I型和IV型胶原的结合能力较低，而对人玻连蛋白、人乳铁蛋白和人血小板反应蛋白的结合能力较高。在托德-休伊特肉汤中培养的细菌对玻连蛋白和血小板反应蛋白的结合能力增强。在颗粒凝集试验（PAA）中，粪肠球菌菌株与包被的乳胶珠强烈反应，而屎肠球菌菌株通常不反应。肠球菌结合细胞外基质蛋白的能力受加热和蛋白酶消化的影响，这表明一些蛋白质结合成分在用蛋白酶处理后会暴露于表面。当细胞与未标记的蛋白质预孵育时，125I标记的蛋白质与粪肠球菌E70菌株的结合受到抑制。用硫酸化聚合物如硫酸葡聚糖（分子量5000和8000）、戊聚糖硫酸酯和肝素预孵育细胞会降低玻连蛋白、乳铁蛋白和血小板反应蛋白的结合。当细菌与半乳糖、岩藻糖和甘露糖胺预孵育时，乳铁蛋白和血小板反应蛋白的结合也会降低，但与甘露糖预孵育时不会。30株粪肠球菌菌株均表现出明显的表面疏水性，但14株屎肠球菌菌株中有10株表现出亲水性细胞表面。
