Xiao J, Höök M, Weinstock G M, Murray B E
Center for the Study of Emerging and Re-emerging Pathogens, Department of Internal Medicine, University of Texas Medical School, Houston 77030, USA.
FEMS Immunol Med Microbiol. 1998 Aug;21(4):287-95. doi: 10.1111/j.1574-695X.1998.tb01176.x.
The adherence of 44 clinical isolates of Enterococcus faecalis, a common cause of endocarditis, and 13 Enterococcus faecium to substrates of six extracellular matrix (ECM) proteins was examined using 35S-labeled bacteria. One E. faecalis strain, isolated from a patient with endocarditis, adhered to collagen types I and IV and another E. faecalis strain adhered to laminin and to collagen types I and IV. However, most isolates showed little adherence ( < 5% of added cells adhered) when grown at 37 degrees C regardless of their source (endocarditis, urine or fecal sample). When grown at 46 degrees C (but not when grown in CO2 or nutrient limited media), most isolates of E. faecalis increased their adherence to immobilized laminin, collagen types I and IV but not to fibronectin, fibrinogen or bovine serum albumin, whereas none of the E. faecium increased adherence when grown at 46 degrees C or 50 degrees C. The adherence of E. faecalis was eliminated by digestion with trypsin, suggesting that a protein is somehow important, directly or indirectly, for adherence to occur. Pre-incubation of bacteria with soluble collagen types I and IV inhibited the adherence to these ECM proteins. These results demonstrate that in E. faecalis, adherence to ECM proteins is produced during routine in vitro growth conditions by occasional isolates and can be produced during certain stressful growth conditions by others. Whether this adherence relates to the propensity of E. faecalis to cause endocarditis remains to be determined.
使用35S标记的细菌检测了44株粪肠球菌（心内膜炎的常见病因）临床分离株和13株屎肠球菌对六种细胞外基质（ECM）蛋白底物的黏附情况。从一名心内膜炎患者分离出的一株粪肠球菌菌株黏附于I型和IV型胶原蛋白，另一株粪肠球菌菌株黏附于层粘连蛋白以及I型和IV型胶原蛋白。然而，大多数分离株在37℃培养时，无论其来源（心内膜炎、尿液或粪便样本），黏附能力都很低（黏附的添加细胞少于5%）。当在46℃培养时（但在二氧化碳培养或营养受限培养基中培养时不出现这种情况），大多数粪肠球菌分离株增加了对固定化层粘连蛋白、I型和IV型胶原蛋白的黏附，但对纤连蛋白、纤维蛋白原或牛血清白蛋白的黏附没有增加，而屎肠球菌在46℃或50℃培养时均未增加黏附能力。粪肠球菌的黏附可被胰蛋白酶消化消除，这表明某种蛋白质直接或间接对黏附的发生具有重要作用。用可溶性I型和IV型胶原蛋白对细菌进行预孵育可抑制其对这些ECM蛋白的黏附。这些结果表明，在粪肠球菌中，偶尔的分离株在常规体外培养条件下会产生对ECM蛋白的黏附，而其他分离株在某些应激生长条件下也会产生这种黏附。这种黏附是否与粪肠球菌引起心内膜炎的倾向有关仍有待确定。
