乳酸乳球菌乳酸亚种中热、酸和紫外线辐射对伴侣蛋白GroEL和共伴侣蛋白GroES的差异诱导作用。

Differential induction of the chaperonin GroEL and the Co-chaperonin GroES by heat, acid, and UV-irradiation in Lactococcus lactis subsp. lactis.

作者信息

Hartke A, Frère J, Boutibonnes P, Auffray Y

机构信息

Laboratoire de Microbiologie de l'Environnement, Université de Caen, France.

出版信息

Curr Microbiol. 1997 Jan;34(1):23-6. doi: 10.1007/s002849900138.

DOI:10.1007/s002849900138

PMID:8939797

Abstract

Microsequencing of a polypeptide with MW of 14.5 and pI of 5.0 induced by heat treatment at 42 degrees C and 50 degrees C in Lactococcus lactis subsp. lactis revealed that it corresponds to the co-chaperonin GroES. Quantitative analysis of analytical 2-D gels showed a relative induction of 12- and 11-fold after 30 min of heat adaptation at 42 degrees C and 50 degrees C, respectively. GroES is also induced by an acid shift from pH 7 to pH 5.5 and by UV254 nm-irradiation, with relative induction factors of 3.8 and 2.3, respectively. To our knowledge this is the first report showing induction of GroES by mild acid treatment. Contrasting to the relative induction of the groEL gene product, the second protein encoded by the groESL operon, GroES shows significantly higher induction under all stress situations.

摘要

对乳酸乳球菌乳酸亚种在42℃和50℃热处理诱导产生的分子量为14.5、等电点为5.0的一种多肽进行微测序，结果表明它与伴侣蛋白GroES相对应。分析型二维凝胶的定量分析显示，在42℃和50℃热适应30分钟后，相对诱导倍数分别为12倍和11倍。GroES也可由pH从7变为5.5的酸转移以及254nm紫外线照射诱导产生，相对诱导因子分别为3.8和2.3。据我们所知，这是首次报道轻度酸处理可诱导GroES产生。与groEL基因产物（groESL操纵子编码的第二种蛋白质）的相对诱导情况相反，GroES在所有应激情况下均显示出明显更高的诱导水平。