巴西固氮螺菌色氨酸合成酶基因（trpE(G)）的分离与特性分析，该基因编码邻氨基苯甲酸合成酶。

Isolation and characterization of the Azospirillum brasilense trpE(G) gene, encoding anthranilate synthase.

作者信息

De Troch P, Dosselaere F, Keijers V, de Wilde P, Vanderleyden J

机构信息

F.A. Janssens Laboratory of Genetics, Department of Applied Plant Sciences, Katholieke Universiteit Leuven, Belgium.

出版信息

Curr Microbiol. 1997 Jan;34(1):27-32. doi: 10.1007/s002849900139.

DOI:10.1007/s002849900139

PMID:8939798

Abstract

The Azospirillum brasilense trpE gene has been isolated by DNA hybridization and by genetic complementation of an Escherichia coli trpE deletion mutant. DNA sequence analysis of a 3.1-kb PstI restriction fragment of A. brasilense revealed the presence of an open reading frame encoding a putative TrpE(G) fusion protein. Previously an A. brasilense clone containing trpGDC was identified (Zimmer et al. Mol Gen Genet 229:41-51, 1991). It can, therefore, be concluded that A. brasilense contains two trpG genes. A putative leader peptide is found upstream of trpE(G), containing three consecutive tryptophan residues. Putative terminator and anti-terminator loops have also been identified. The LLESX10S motif, which is responsible for feedback inhibition by tryptophan in other TrpE proteins, is absent in the A. brasilense TrpE(G) fused protein.

摘要

通过DNA杂交以及对大肠杆菌trpE缺失突变体的遗传互补作用，已分离出巴西固氮螺菌的trpE基因。对巴西固氮螺菌一个3.1 kb PstI限制片段的DNA序列分析表明，存在一个编码假定TrpE(G)融合蛋白的开放阅读框。此前已鉴定出一个含有trpGDC的巴西固氮螺菌克隆（齐默等人，《分子遗传学与普通遗传学》229:41 - 51，1991年）。因此，可以得出结论，巴西固氮螺菌含有两个trpG基因。在trpE(G)上游发现了一个假定的前导肽，其中含有三个连续的色氨酸残基。还鉴定出了假定的终止环和抗终止环。在巴西固氮螺菌TrpE(G)融合蛋白中不存在负责色氨酸对其他TrpE蛋白进行反馈抑制的LLESX10S基序。