Suppr超能文献

铜绿假单胞菌色氨酸途径四个早期基因的DNA序列及特征分析

DNA sequences and characterization of four early genes of the tryptophan pathway in Pseudomonas aeruginosa.

作者信息

Essar D W, Eberly L, Han C Y, Crawford I P

机构信息

Microbiology Department, University of Iowa, Iowa City 52242.

出版信息

J Bacteriol. 1990 Feb;172(2):853-66. doi: 10.1128/jb.172.2.853-866.1990.

Abstract

Two pairs of related but easily distinguishable genes for the two subunits of anthranilate synthase have been identified in Pseudomonas aeruginosa. These were cloned, sequenced, inactivated in vitro by insertion of an antibiotic resistance cassette, and returned to the P. aeruginosa chromosome, replacing the wild-type gene. Gene replacement implicated only one of the pairs in tryptophan biosynthesis. This report describes the cloning and sequencing of the tryptophan-related gene pair, designated trpE and trpG, and presents experiments implicating their gene products in tryptophan production. DNA sequence analysis as well as growth and enzyme assays of insertionally inactivated strains indicated that trpG is the first gene in a three-gene operon that also includes trpD and trpC. Complementation of Trp auxotrophs by R-prime plasmids (T. Shinomiya, S. Shiga, and M. Kageyama, Mol. Gen. Genet., 189:382-389, 1983) has shown that a large cluster of pyocin R2 genes is flanked at one end by trpE and the other end by trpDC; the physical map that was obtained shows the distance between trpE and trpDC to be about 25 kilobases. Our restriction map of the trpE and trpGDC regions agrees with data presented by Shinomiya et al.

摘要

在铜绿假单胞菌中已鉴定出与邻氨基苯甲酸合酶两个亚基相关但易于区分的两对基因。将这些基因进行克隆、测序,通过插入抗生素抗性盒在体外使其失活,然后再导入铜绿假单胞菌染色体,取代野生型基因。基因置换表明只有其中一对基因参与色氨酸的生物合成。本报告描述了与色氨酸相关的基因对(命名为trpE和trpG)的克隆和测序,并展示了证明其基因产物参与色氨酸产生的实验。对插入失活菌株的DNA序列分析以及生长和酶活性测定表明,trpG是一个三基因操纵子中的第一个基因,该操纵子还包括trpD和trpC。R-prime质粒对色氨酸营养缺陷型的互补作用(T. Shinomiya、S. Shiga和M. Kageyama,《分子与普通遗传学》,189:382 - 389,1983)表明,一大簇绿脓菌素R2基因一端侧翼为trpE,另一端侧翼为trpDC;所获得的物理图谱显示trpE与trpDC之间的距离约为25千碱基。我们绘制的trpE和trpGDC区域的限制性图谱与Shinomiya等人提供的数据一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2216/208515/caecf7b7f999/jbacter01044-0362-a.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验