Stillman B
Cold Spring Harbor Laboratory, P.O. Box 100, Cold Spring Harbor, NY 11724, USA.
Science. 1996 Dec 6;274(5293):1659-64. doi: 10.1126/science.274.5293.1659.
The initiation of DNA replication in eukaryotic cells is a highly regulated process that leads to the duplication of the genetic information for the next cell generation. This requires the ordered assembly of many proteins at the origins of DNA replication to form a competent, pre-replicative chromosomal state. In addition to this competent complex, at least two cell cycle regulated protein kinase pathways are required to affect a transition to a post-replicative chromosomal state. Protein kinases required to establish mitosis prevent re-replication of the DNA. As cells exit mitosis, the cell cycle is reset, allowing the establishment of a new, competent replication state.
真核细胞中DNA复制的起始是一个高度调控的过程，它导致遗传信息复制以传递给下一代细胞。这需要许多蛋白质在DNA复制起点有序组装，以形成一个有活性的、复制前的染色体状态。除了这个有活性的复合物外，至少还需要两条受细胞周期调控的蛋白激酶途径来促使向复制后染色体状态转变。启动有丝分裂所需的蛋白激酶可防止DNA再次复制。当细胞退出有丝分裂时，细胞周期被重置，从而允许建立一个新的、有活性的复制状态。
