Gulati S, McQuillen D P, Mandrell R E, Jani D B, Rice P A
Maxwell Finland Laboratory for Infectious Diseases, Boston Medical Center, Boston University School of Medicine, Massachusetts 02118, USA.
J Infect Dis. 1996 Dec;174(6):1223-37. doi: 10.1093/infdis/174.6.1223.
Natural infection with Neisseria gonorrhoeae may elicit a substantial antibody response directed against gonococcal lipooligosaccharide. Monoclonal antibody (MAb) 2C7 recognized a gonococcal lipooligosaccharide epitope, identified the epitope directly in 94% of 68 consecutive culture-positive genital secretions, and recognized 95% of 101 randomly chosen fresh (second-passage) gonococcal isolates. The epitope was stably maintained after multiple in vitro passages and did not compete with any of the known cross-reactive human glycosphingolipid structures. MAb 2C7 mediated in vitro killing and phagocytosis by human polymorphonuclear leukocytes of 1 serum-sensitive (sialylated or not) and 1 stably serum-resistant gonococcal isolate that expressed the epitope. Gonococcal endometritis and disseminated infection elicited increases (6.5-fold IgM, 4.4-fold IgG; 18-fold IgM, 17-fold IgG, respectively) in anti-2C7 epitope antibody. Immunization with a gonococcal outer membrane vaccine elicited a mean 44.5-fold increase in IgG anti-2C7 epitope antibody in 20 of 28 subjects. The epitope identified by MAb 2C7 may represent an excellent target for a potentially protective gonococcal vaccine candidate.
淋球菌自然感染可能引发针对淋球菌脂寡糖的大量抗体反应。单克隆抗体（MAb）2C7识别一种淋球菌脂寡糖表位，在连续68份培养阳性的生殖器分泌物中有94%可直接鉴定出该表位，并且能识别101株随机选取的新鲜（传代两次）淋球菌分离株中的95%。该表位在多次体外传代后能稳定维持，且不与任何已知的交叉反应性人糖鞘脂结构竞争。MAb 2C7介导人多形核白细胞对1株表达该表位的血清敏感（唾液酸化或未唾液酸化）和1株稳定血清抗性淋球菌分离株的体外杀伤和吞噬作用。淋球菌性子宫内膜炎和播散性感染使抗2C7表位抗体增加（IgM分别增加6.5倍、IgG增加4.4倍；IgM分别增加18倍、IgG增加17倍）。用淋球菌外膜疫苗免疫28名受试者中的20名，使抗2C7表位抗体的IgG平均增加44.5倍。MAb 2C7识别的表位可能是一种潜在的保护性淋球菌疫苗候选物的极佳靶点。
