Gulati S, McQuillen D P, Sharon J, Rice P A
Department of Medicine, Boston Medical Center, Massachusetts 02118, USA.
J Infect Dis. 1996 Dec;174(6):1238-48. doi: 10.1093/infdis/174.6.1238.
An anti-idiotope monoclonal antibody (MAb), called CA1 (Ab2), was produced in mice against MAb 2C7, which recognizes a widely in vivo-expressed gonococcal lipooligosaccharide (LOS) epitope. Mice immunized with MAb CA1 initially had a 2.5-fold increase in IgG (12-fold after a booster) but no increase in IgM anti-LOS (Ab1') antibody. Control mice immunized with LOS had a 4.5-fold rise in IgG and 4-fold rise in IgM anti-LOS antibody. In rabbits, MAb CA1 elicited a 9-fold rise in IgG and a 3.3-fold rise in IgM anti-LOS (Ab1') antibody. Ab1' antibody bactericidal activity was 1-2 logs greater than that produced by immunization with LOS. Ab1' mediated complete human polymorphonuclear leukocyte phagocytosis of 2C7 epitope-positive (but not 2C7 epitope-negative) gonococci. MAb CA1 acts as a molecular surrogate (Ab2beta) for the nominal LOS antigen and may form the basis for vaccine candidates for human immunization against Neisseria gonorrhoeae.
一种名为CA1（Ab2）的抗独特型单克隆抗体在小鼠体内针对单克隆抗体2C7产生，2C7可识别一种在体内广泛表达的淋球菌脂寡糖（LOS）表位。用单克隆抗体CA1免疫的小鼠最初IgG增加了2.5倍（加强免疫后增加12倍），但抗LOS（Ab1'）抗体的IgM没有增加。用LOS免疫的对照小鼠IgG增加了4.5倍，抗LOS抗体的IgM增加了4倍。在兔子中，单克隆抗体CA1使抗LOS（Ab1'）抗体的IgG增加了9倍，IgM增加了3.3倍。Ab1'抗体的杀菌活性比用LOS免疫产生的杀菌活性高1至2个对数。Ab1'介导了对2C7表位阳性（但不是2C7表位阴性）淋球菌的完全人类多形核白细胞吞噬作用。单克隆抗体CA1作为名义LOS抗原的分子替代物（Ab2β），可能构成用于人类免疫预防淋病奈瑟菌的候选疫苗的基础。
