Kawauchi S, Fukuda T, Tsuneyoshi M
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ultrastruct Pathol. 1996 Nov-Dec;20(6):549-61. doi: 10.3109/01913129609016359.
A new immunoelectron microscopy procedure was developed by remaking the fixed-frozen tissue specimens into LR White resin blocks suitable for postembedding colloidal gold immunolabeling, and used to examine 16 cases of small round cell soft tissue sarcomas. In rhabdomyosarcoma, ultrastructural double-immunogold staining demonstrated a coexpression of muscle specific actin and desmin in the same tumor cell. In both Ewing's sarcoma and peripheral neuroepithelioma, the heterogeneous expression of MIC2 gene product (p30/32MIC2) in each tumor cell was demonstrated as well. In peripheral neuroepithelioma, the colloidal gold immunolabeling for neurofilament demonstrated the intermediate filaments surrounding microtubules. The procedure for ultrastructural colloidal gold immunolabeling using fixed-frozen tissue is thus considered to be useful not only for tumor diagnosis, but also for investigating various subcellular structures.
通过将固定冷冻的组织标本制成适合包埋后胶体金免疫标记的LR White树脂块，开发了一种新的免疫电子显微镜方法，并用于检查16例小圆细胞软组织肉瘤。在横纹肌肉瘤中，超微结构双免疫金染色显示同一肿瘤细胞中肌肉特异性肌动蛋白和结蛋白共表达。在尤因肉瘤和外周神经上皮瘤中，也证实了每个肿瘤细胞中MIC2基因产物（p30/32MIC2）的异质性表达。在外周神经上皮瘤中，神经丝的胶体金免疫标记显示中间丝围绕微管。因此，使用固定冷冻组织的超微结构胶体金免疫标记方法不仅被认为对肿瘤诊断有用，而且对研究各种亚细胞结构也有用。
