Arai H, Satoh K, Terajima M, Nakagawa T, Higuchi M, Kosaka Y, Zhu C, Sasaki H
Department of Geriatric Medicine, Tohoku University School of Medicine.
Nihon Ronen Igakkai Zasshi. 1996 Sep;33(9):669-75. doi: 10.3143/geriatrics.33.669.
Levels of the microtubule-associated protein tau in cerebrospinal fluid (CSF-tau) were measured in samples from 87 patients with Alzheimer's disease (AD), 114 patients with non-AD neurological diseases, and 22 normal control subjects, by sandwich enzyme-linked immunosorbent assay. The CSF-tau level was significantly higher in patients with AD than in patients with non-AD neurological diseases and in controls. High CSF-tau levels were found irrespective of age at onset, apolipoprotein E genotype, clinical stage, and ethnic group. Western blots of AD CSF proteins revealed two to three immunoreactive bands with apparent molecular mass between 50 and 65 kDa, which is consistent with phosphorylated CSF-tau. These results suggest that CSF-tau reflects progressive accumulation of tau due to the progressive death of neurons in the AD brain. Assay of CSF-tau may prove to be a reliable diagnostic test for AD.
通过夹心酶联免疫吸附测定法，对87例阿尔茨海默病（AD）患者、114例非AD神经疾病患者和22名正常对照者的样本进行了脑脊液中微管相关蛋白tau水平（CSF-tau）的检测。AD患者的CSF-tau水平显著高于非AD神经疾病患者和对照组。无论发病年龄、载脂蛋白E基因型、临床分期和种族如何，均发现CSF-tau水平较高。AD脑脊液蛋白的免疫印迹显示出两到三条免疫反应带，表观分子量在50至65 kDa之间，这与磷酸化的CSF-tau一致。这些结果表明，CSF-tau反映了AD大脑中神经元逐渐死亡导致的tau蛋白的渐进性积累。CSF-tau检测可能被证明是一种可靠的AD诊断测试。
