Chakir J, Laviolette M, Boutet M, Laliberté R, Dubé J, Boulet L P
Unité de Recherche, Centre de Pneumologie de l'Hôpital Laval, Université Laval, Sainte-Foy, Québec, Canada.
Lab Invest. 1996 Nov;75(5):735-44.
We analyzed by immunohistochemistry the distribution of types I, II, III, IV, V, and VII collagens, laminin, and fibronectin in the bronchial biopsy specimens of nonasthmatic subjects with seasonal allergic rhinitis (n = 8) and compared these results with those found in mild stable allergic asthmatics (n = 6) and normal controls (n = 5). The content of type I and III collagens was increased in rhinitic subjects compared with controls. These collagens were focally deposited in the reticular basement membrane area. Three subjects with allergic rhinitis had no fibronectin deposition in their basement membrane, as in controls, whereas the other five had a focal fibronectin deposition. In asthmatic patients, type I and III collagens and fibronectin were more abundant and more uniformly distributed underneath the basement membrane than they were in rhinitic subjects. Expression of type II, IV, V, and VII collagens and laminin were similar in the three groups. Electron microscopic and immunohistochemical analyses of bronchial mucosa showed a network of myofibroblasts beneath the epithelium in rhinitis as in asthma subjects. These data show that the irregularly distributed subepithelial fibrosis observed in subjects with allergic rhinitis results from the deposition of type I and III collagens and fibronectin, probably produced by bronchial myofibroblasts. These results suggest the presence of an active structural remodeling in the lower airways of allergic rhinitic subjects that is similar in nature to that seen in asthma, although less marked.
我们通过免疫组织化学分析了患有季节性变应性鼻炎的非哮喘受试者（n = 8）支气管活检标本中I型、II型、III型、IV型、V型和VII型胶原蛋白、层粘连蛋白和纤连蛋白的分布，并将这些结果与轻度稳定型变应性哮喘患者（n = 6）和正常对照者（n = 5）的结果进行了比较。与对照组相比，鼻炎患者I型和III型胶原蛋白的含量增加。这些胶原蛋白局灶性沉积在网状基底膜区域。三名变应性鼻炎患者的基底膜中没有纤连蛋白沉积，与对照组一样，而其他五名患者有局灶性纤连蛋白沉积。在哮喘患者中，I型和III型胶原蛋白以及纤连蛋白在基底膜下方比在鼻炎患者中更丰富且分布更均匀。II型、IV型、V型和VII型胶原蛋白以及层粘连蛋白的表达在三组中相似。支气管黏膜的电子显微镜和免疫组织化学分析显示，与哮喘患者一样，鼻炎患者上皮下有肌成纤维细胞网络。这些数据表明，变应性鼻炎患者中观察到的不规则分布的上皮下纤维化是由I型和III型胶原蛋白以及纤连蛋白的沉积引起的，可能由支气管肌成纤维细胞产生。这些结果表明，变应性鼻炎患者下呼吸道存在活跃的结构重塑，其性质与哮喘相似，尽管不太明显。
