Vincent M J, Novembre F J, Yamshchikov V F, McClure H M, Compans R W
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Virology. 1996 Dec 1;226(1):57-65. doi: 10.1006/viro.1996.0627.
We report the isolation of a virus from a baboon imported from Kenya and the analysis of the nucleotide sequence of the env gene. Comparison of the complete nucleotide sequence of the env gene of different HTLV-1 strains and the baboon T-cell leukemia virus (designated BTLV) indicated similarities ranging from 92.5 to 97.4%. In contrast, only 89.1% similarity was observed between the BTLV env sequence and that of simian T-cell leukemia virus (PtM3). The sequences corresponding to the glycosylation sites, endoproteolytic processing site, and major immunological determinants were strictly conserved between BTLV and HTLV-1. To characterize the expressed protein we used a vaccinia expression system, which indicated that a protein of 62 kDa is encoded by the envelope gene. The protein acquired mostly high mannose modifications and was localized predominantly in the endoplasmic reticulum. A fraction of the protein was expressed at the cell surface, where it could induce membrane fusion of target cells. The existence of HTLV-1-like viruses in baboons indicates the potential risk of transmission of such virus from these nonhuman primates to humans, thus highlighting the need for specific screening for such viruses during xenotransplantation.
我们报告了从一只从肯尼亚进口的狒狒身上分离出一种病毒，并对其env基因的核苷酸序列进行了分析。不同HTLV-1毒株的env基因完整核苷酸序列与狒狒T细胞白血病病毒（命名为BTLV）的比较显示，相似性范围在92.5%至97.4%之间。相比之下，BTLV的env序列与猿猴T细胞白血病病毒（PtM3）的序列之间仅观察到89.1%的相似性。BTLV和HTLV-1之间对应于糖基化位点、内切蛋白水解加工位点和主要免疫决定簇的序列严格保守。为了表征所表达的蛋白质，我们使用了痘苗表达系统，该系统表明包膜基因编码一种62 kDa的蛋白质。该蛋白质大多获得了高甘露糖修饰，并且主要定位于内质网。一部分蛋白质在细胞表面表达，在那里它可以诱导靶细胞的膜融合。狒狒中存在HTLV-1样病毒表明这种病毒从这些非人类灵长类动物传播给人类的潜在风险，从而突出了在异种移植期间对此类病毒进行特异性筛查的必要性。
