Shao L, Shinzawa H, Ishikawa K, Zhang X, Ishibashi M, Misawa H, Yamada N, Togashi H, Takahashi T
Second Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Biochem Biophys Res Commun. 1996 Nov 21;228(3):785-91. doi: 10.1006/bbrc.1996.1732.
The nucleotide sequence of hepatitis G virus (HGV) genome was determined by analysis of cDNA clones obtained by long reverse transcription-polymerase chain reaction (long RT PCR) and 5'- and 3'-rapid amplification of cDNA ends (RACE) from a Japanese patient (Iw) with non A-E hepatitis. The HGV-Iw genome, consisting of 9375 nucleotides, contains a long open reading frame encoding 2873 amino acid residues. Comparison of HGV-Iw with two American isolates of HGV and one African isolate of GB virus C (GBV-C) indicates that although the nucleotide sequences of these isolates were considerably divergent (86.2% to 93.3%), the deduced amino acid sequences shared an extremely high degree of identity (96.1% to 100%). It was also found that HGV-Iw was more closely related to the HGV isolates from USA than to the GBV-C isolate from Africa.
通过对一位患有非甲 - 戊型肝炎的日本患者（Iw）的长链逆转录 - 聚合酶链反应（长链RT PCR）以及5'和3' cDNA末端快速扩增（RACE）获得的cDNA克隆进行分析，确定了庚型肝炎病毒（HGV）基因组的核苷酸序列。HGV - Iw基因组由9375个核苷酸组成，包含一个编码2873个氨基酸残基的长开放阅读框。将HGV - Iw与两株美国HGV分离株以及一株非洲GB病毒C（GBV - C）分离株进行比较，结果表明，尽管这些分离株的核苷酸序列差异很大（86.2%至93.3%），但推导的氨基酸序列具有极高的同一性（96.1%至100%）。还发现HGV - Iw与来自美国的HGV分离株的关系比与来自非洲的GBV - C分离株的关系更为密切。
