Carreno M P, Stuard S, Bonomini M, Settefrati N, Tetta C, Albertazzi A, Haeffner-Cavaillon N
Institut National de la Santé et de la Recherche Médicale (INSERM U430), Hôpital Broussais, Paris, France.
Nephrol Dial Transplant. 1996 Nov;11(11):2248-57. doi: 10.1093/oxfordjournals.ndt.a027144.
Transient nature of adhesive interactions occurring during cell margination is mainly dependent on expression of selectins which are shed by activated cells. This shedding in the circulation may play an important role as anti-inflammatory mediator. Haemodialysis is also associated with P-selectin (CD62P)/sialyl-Lewis(x) (CD15s) interactions which mediate platelet-leukocyte coaggregation. We further investigated the mechanisms underlying leukocyte margination during haemodialysis.
CD15s, CD11b and CD61 expression on circulating leukocytes from patients dialysed on synthetic membranes (modified polyacrylonitrile (SPAN), polysulphone (PS), and polyacrylonitrile (AN69) was assessed by cytofluorometry in a prospective crossover trial. We measured plasma levels C3a/C3a desArg, soluble CD62P, and CD62E molecules obtained from patients and healthy individuals.
Expression of CD11b and CD15s was upregulated on neutrophils from patients dialysed with SPAN and PS membranes during the dialysis session. A significant negative correlation was found between the expression of CD11b or CD15s molecules and neutrophil counts as well as between CD15s expression and monocyte counts during haemodialysis. As assessed by CD61 expression on leukocytes, we observed that platelets bound significantly onto both neutrophils and monocytes during dialysis with both membranes. A significant positive correlation was found between the expression of CD11b molecules and the percentage of CD61+ monocytes counts during SPAN and PS dialysis. We found a significant increase of soluble CD62P in plasma samples obtained from haemodialysed patients before the dialysis session as compared to the levels detected in plasma from healthy individuals.
This study documents a major role of CD15s, CD11b, CD61, CD62P molecules in the transient leukocytes activation and margination during haemodialysis on synthetic membranes despite their low complement-activating properties.
细胞边缘化过程中发生的黏附相互作用具有短暂性，这主要取决于选择素的表达，而选择素由活化细胞脱落。这种在循环中的脱落可能作为抗炎介质发挥重要作用。血液透析还与介导血小板 - 白细胞共聚集的P - 选择素（CD62P）/唾液酸化路易斯x（CD15s）相互作用有关。我们进一步研究了血液透析过程中白细胞边缘化的潜在机制。
在一项前瞻性交叉试验中，通过细胞荧光测定法评估了在合成膜（改性聚丙烯腈（SPAN）、聚砜（PS）和聚丙烯腈（AN69））上进行透析的患者循环白细胞上CD15s、CD11b和CD61的表达。我们测量了来自患者和健康个体的血浆中C3a/C3a去精氨酸、可溶性CD62P和CD62E分子的水平。
在透析过程中，使用SPAN和PS膜进行透析的患者中性粒细胞上CDllb和CD15s的表达上调。在血液透析期间，发现CD11b或CD15s分子的表达与中性粒细胞计数之间以及CD15s表达与单核细胞计数之间存在显著的负相关。通过白细胞上的CD61表达评估，我们观察到在使用两种膜进行透析期间，血小板与中性粒细胞和单核细胞均有显著结合。在SPAN和PS透析期间，发现CD11b分子的表达与CD61 +单核细胞计数的百分比之间存在显著正相关。与健康个体血浆中检测到的水平相比，我们发现血液透析患者透析前采集的血浆样本中可溶性CD62P显著增加。
本研究证明了CD15s、CD11b、CD61、CD62P分子在合成膜血液透析过程中短暂的白细胞激活和边缘化中起主要作用，尽管它们的补体激活特性较低。
