响尾蛇科蛇毒腺丝氨酸蛋白酶的加速进化

Accelerated evolution of crotalinae snake venom gland serine proteases.

作者信息

Deshimaru M, Ogawa T, Nakashima K, Nobuhisa I, Chijiwa T, Shimohigashi Y, Fukumaki Y, Niwa M, Yamashina I, Hattori S, Ohno M

机构信息

Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.

出版信息

FEBS Lett. 1996 Nov 11;397(1):83-8. doi: 10.1016/s0014-5793(96)01144-1.

DOI:10.1016/s0014-5793(96)01144-1

PMID:8941719

Abstract

Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.

摘要

从竹叶青蛇（饭匙倩蛇）和草原蝰蛇（绿竹叶青蛇）毒腺cDNA文库中分离出的8个编码丝氨酸蛋白酶的cDNA显示，在成熟蛋白编码区积累了非同义核苷酸替换，导致氨基酸发生变化。使用两种合适的探针对竹叶青蛇基因组DNA进行Southern印迹分析表明，毒腺丝氨酸蛋白酶基因在基因组中形成了一个多基因家族。这些观察结果表明，毒腺丝氨酸蛋白酶的氨基酸序列以加速的方式多样化。由于此前在蝰蛇科蛇毒腺磷脂酶A2（PLA2）同工酶基因中也发现了类似特征，加速进化似乎在蝰蛇科蛇毒腺的多个同工酶家族中普遍存在。