Komorowski R C, Torneck C D, Hu J W
University of Toronto, Faculty of Dentistry, Ontario, Canada.
J Endod. 1996 Aug;22(8):414-7. doi: 10.1016/S0099-2399(96)80242-1.
This study investigated possible collateral C-fiber innervation between the pulps of rat molars by assessing neurogenic inflammation (NI) induced by the C-fiber excitant mustard oil (MO). MO was applied to the pulp of the left mandibular first molar in two groups of rats: group 1, guanethidine sympathectomized rats (to dismiss sympathetic activation by MO); and group 2, unsympathectomized rats. A third group of unsympathectomized rats (group 3) had saline applied to the pulp of the left mandibular molar and served as a MO control. The NI-related plasma extravasation was examined in these teeth and in the remaining left mandibular teeth by a spectrophotometric analysis of extravasated plasma protein bound to Evans' Blue (EB) dye. The collateral innervation pattern was inferred from the NI pattern. EB concentrations were measured in the left mandibular teeth and the corresponding contralateral teeth, and expressed as a ratio. Statistical analysis of the data revealed significant differences in EB ratios in the first, second, and third molars between groups 1 and 3. This result suggests collateral C-fiber innervation exists within the pulps of molar teeth in the same dental quadrant. No difference in EB ratios was noted in the first and second molars between groups 1 and 2. Therefore, sympathetic efferents have no apparent effect on the degree of MO-induced NI.
本研究通过评估C纤维兴奋剂芥子油（MO）诱导的神经源性炎症（NI），调查大鼠磨牙牙髓之间可能存在的C纤维侧支神经支配。将MO应用于两组大鼠的左下颌第一磨牙牙髓：第1组，胍乙啶交感神经切除术大鼠（以消除MO引起的交感神经激活）；第2组，未进行交感神经切除术的大鼠。第三组未进行交感神经切除术的大鼠（第3组）将生理盐水应用于左下颌磨牙牙髓，并作为MO对照组。通过对与伊文思蓝（EB）染料结合的外渗血浆蛋白进行分光光度分析，检查这些牙齿和其余左下颌牙齿中与NI相关的血浆外渗情况。从NI模式推断侧支神经支配模式。测量左下颌牙齿和相应对侧牙齿中的EB浓度，并表示为比率。对数据的统计分析显示，第1组和第3组之间第一、第二和第三磨牙中的EB比率存在显著差异。该结果表明，同一牙象限的磨牙牙髓内存在C纤维侧支神经支配。第1组和第2组之间第一和第二磨牙的EB比率没有差异。因此，交感神经传出纤维对MO诱导的NI程度没有明显影响。
