Moe Kyaw, Kettunen Päivi, Kvinnsland Inger Hals, Luukko Keijo
Department of Biomedicine, Faculty of Medicine and Dentistry, University of Bergen, Jonas Lies vei 91, N-5009 Bergen, Norway.
Arch Oral Biol. 2008 Sep;53(9):865-73. doi: 10.1016/j.archoralbio.2008.03.004. Epub 2008 Apr 23.
Our goal was to study the development of pioneer sympathetic innervation of dental pulp of mouse mandibular first molar.
We used double fluorescent immunohistochemistry with tyrosine hydroxylase (TH) and anti-medium-chain neurofilament (2H3) antibodies to detect sympathetic and sensory nerve fibres. Serial sections of whole teeth from postnatal days (PN) 0-14, trigeminal and sympathetic superior cervical ganglia of PN 15 mice were examined with confocal microscope.
There were two main findings. The unexpected finding was that 2H3 antibody was specific only for sensory nerve fibres and neurons and failed to stain either sympathetic nerve fibres or neurons. The main finding was that although both sympathetic and sensory nerve fibres were already seen near the tooth germ at the newborn stage, the pioneer sympathetic nerve fibres were first observed in the dental pulp only after the onset of root formation on day 9, in contrast to sensory nerve fibres which entered the tooth already on day 4.
Pioneer sympathetic innervation of dental pulp starts on postnatal day 9 and follows sensory innervation. This indicates differential developmental regulation of the initial sensory and sympathetic innervation of teeth and provides essential background data for further studies on the molecular regulation of pulp innervation.
我们的目标是研究小鼠下颌第一磨牙牙髓的先驱性交感神经支配的发育情况。
我们使用酪氨酸羟化酶（TH）和抗中链神经丝（2H3）抗体进行双重荧光免疫组织化学，以检测交感神经和感觉神经纤维。用共聚焦显微镜检查出生后0至14天全牙的连续切片、出生后15天小鼠的三叉神经节和交感神经颈上神经节。
有两个主要发现。意外发现是2H3抗体仅对感觉神经纤维和神经元具有特异性，对交感神经纤维或神经元均无染色。主要发现是，尽管在新生阶段牙胚附近已可见交感神经和感觉神经纤维，但先驱性交感神经纤维直到第9天牙根形成开始后才首次在牙髓中被观察到，而感觉神经纤维在第4天就已进入牙齿。
牙髓的先驱性交感神经支配始于出生后第9天，晚于感觉神经支配。这表明牙齿初始感觉神经和交感神经支配的发育调控存在差异，并为牙髓神经支配分子调控的进一步研究提供了重要的背景数据。
