Senpuku H, Nakai M, Koga T, Hanada N, Nisizawa T
Department of Oral Science, National Institute of Health, Tokyo, Japan.
Oral Microbiol Immunol. 1996 Apr;11(2):121-8. doi: 10.1111/j.1399-302x.1996.tb00346.x.
This study determined the antigen determinants of a 190-kDa protein antigen of Streptococcus mutans that is involved in the initial attachment to the tooth surface. In 5 subjects, the reactivities of serum antibodies to 7 overlapping surface protein antigen fragments covering the entire antigen molecule and 19 sequential overlapping synthetic 19-mer peptides covering the entire A-region of the surface protein antigen were examined with enzyme-linked immunosorbent assay (ELISA). The study showed that the A-region of the antigen is strongly immunogenic in humans and contains several widely distributed epitopes. In addition, an amino acid sequence of the one of dominant epitopes in a certain subject was identified as LTAENTAI with ELISA inhibition assays using the relevant truncated peptides. This epitope was located both at the positions from L-346 to I-364 and L-430 to E-437 of the antigen molecule, and serum antibodies against the epitope were found in 3 of the 5 subjects.
本研究确定了变形链球菌一种190 kDa蛋白质抗原的抗原决定簇，该抗原参与了对牙齿表面的初始附着。对5名受试者，采用酶联免疫吸附测定（ELISA）检测了血清抗体与覆盖整个抗原分子的7个重叠表面蛋白抗原片段以及覆盖表面蛋白抗原整个A区的19个连续重叠合成19肽的反应性。研究表明，该抗原的A区在人类中具有强免疫原性，且包含多个广泛分布的表位。此外，使用相关截短肽通过ELISA抑制试验确定了某一受试者中一个优势表位的氨基酸序列为LTAENTAI。该表位位于抗原分子的L-346至I-364位以及L-430至E-437位，5名受试者中有3名受试者体内发现了针对该表位的血清抗体。
