Yoshio H, Yamada M, Nii S
Department of Neonatology, Okayama National Hospital, Japan.
Acta Paediatr Jpn. 1996 Oct;38(5):429-33. doi: 10.1111/j.1442-200x.1996.tb03521.x.
In order to make a rapid and accurate diagnosis of respiratory syncytial virus (RSV) infection, nasal swabs obtained from 14 neonates suspected of having this disease were examined for the presence of RSV genome by reverse transcription (RT) and nested polymerase chain reaction (PCR) amplification, along with enzyme immunoassay (EIA), serum neutralization testing and virus isolation. The RT-PCR method was sensitive enough to detect a 0.1 50% tissue culture infectious dose (TCID50) per milliliter by nested PCR. The RSV antigen was detected from the samples at more than 100 TCID50 per milliliter by EIA. Nine patients were positive for the presence of RSV genome by first PCR on the day of admission, and eight were also positive by nested PCR even on the fifth hospital day. Among nine PCR positives, four patients were positive for EIA and five for virus isolation. No cases were serologically diagnosed. The cases that were negative for RT-PCR were also negative according to the other methods. In the clinical setting, the RT-PCR assay is more useful for diagnosis of RSV infection than other methods when the suspected cases are negative by EIA assay.
为了快速准确地诊断呼吸道合胞病毒（RSV）感染，对14例疑似患有该病的新生儿采集鼻拭子，通过逆转录（RT）和巢式聚合酶链反应（PCR）扩增检测RSV基因组的存在，并同时进行酶免疫测定（EIA）、血清中和试验和病毒分离。RT-PCR方法足够灵敏，通过巢式PCR能够检测到每毫升0.1 50%组织培养感染剂量（TCID50）。通过EIA从样本中检测到每毫升超过100 TCID50的RSV抗原。9例患者入院当天首次PCR检测RSV基因组呈阳性，8例甚至在住院第5天巢式PCR检测仍为阳性。在9例PCR阳性患者中，4例EIA检测呈阳性，5例病毒分离呈阳性。无病例通过血清学诊断。RT-PCR检测为阴性的病例，其他方法检测也为阴性。在临床环境中，当疑似病例EIA检测为阴性时，RT-PCR检测对于RSV感染的诊断比其他方法更有用。
