Suda H, Moroi C, Inada K, Chida S, Yoshida M
Department of Neonatology, Morioka Red Cross Hospital, Japan.
Acta Paediatr Jpn. 1996 Oct;38(5):444-8. doi: 10.1111/j.1442-200x.1996.tb03524.x.
Endotoxin in both amniotic fluid and cord blood was measured to detect intra-amniotic fetal infection. Both amniotic fluid and cord blood plasma were pretreated by a perchloric acid treatment, and the endotoxin level was measured by Endospecy test. Cut off values for endotoxin in amniotic fluid and cord blood were 8.5 pg/mL and 7.6 pg/mL, respectively. Escherichia coli intra-amniotic infection caused respiratory distress syndrome (RDS)-mimicking pneumonia. Abnormally high values of endotoxin in both amniotic fluid and cord blood were detected. Intra-amniotic infection caused by Gram-positive bacteria (group B streptococci, Enterococcus fecalis) was shown to be endotoxin negative in both amniotic fluid and cord blood. In cases of negative amniotic fluid culture, measurement of the value of endotoxin in the amniotic fluid is useful in identifying intra-amniotic fetal infection.
检测羊水和脐血中的内毒素以检测羊膜腔内胎儿感染。羊水和脐血血浆均经高氯酸处理进行预处理，内毒素水平通过Endospecy检测法进行测量。羊水和脐血中内毒素的临界值分别为8.5 pg/mL和7.6 pg/mL。羊膜腔内大肠杆菌感染可导致类似呼吸窘迫综合征（RDS）的肺炎。检测到羊水和脐血中的内毒素值异常高。由革兰氏阳性菌（B族链球菌、粪肠球菌）引起的羊膜腔内感染在羊水和脐血中均显示内毒素阴性。在羊水培养阴性的情况下，测量羊水中内毒素的值有助于识别羊膜腔内胎儿感染。
